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We found that tonsil but not blood leukocytes were responsive to S1P gradients, suggesting that T cell responsiveness is regulated during their recirculation in vivo. Tonsil naive T cells were readily chemoattracted by S1P in an FTY720-sensitive, S1PR1-dependent manner. Surprisingly, S1P had the opposite effect on effector memory T cells, resident memory T cells, and recently activated T cells, inhibiting their spontaneous or chemokine-induced migration. This inhibition was also more pronounced for CD4 T cells than for CD8 T cell subsets, and was dependent on S1PR2, as shown using the S1PR2 antagonist JTE-013. S1PR1 was progressively downregulated during T cell differentiation whereas S1PR2 expression remained stable. Our results suggest that the ratio between S1PR1 and S1PR2 governs the migratory behavior of T cell subsets. They also challenge previous models of the role of S1P in lymphocyte recirculation and suggest that S1P promotes retention of memory T cell subsets in secondary lymphoid organs, via S1PR2.<\/jats:p>","DOI":"10.4049\/jimmunol.1701278","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T15:50:22Z","timestamp":1513180222000},"page":"551-557","source":"Crossref","is-referenced-by-count":35,"title":["Human Naive and Memory T Cells Display Opposite Migratory Responses to Sphingosine-1 Phosphate"],"prefix":"10.1093","volume":"200","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7188-7655","authenticated-orcid":false,"given":"Annabelle","family":"Drouillard","sequence":"first","affiliation":[{"name":"International Center for Infectiology Research , 69000 Lyon,","place":["France"]},{"name":"INSERM, U1111 , 69000 Lyon,","place":["France"]},{"name":"\u00c9cole Normale Sup\u00e9rieure de Lyon , 69000 Lyon,","place":["France"]},{"name":"Universit\u00e9 Lyon 1 , 69000 Lyon,","place":["France"]},{"name":"CNRS, UMR5308 , 69000 Lyon,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoinette","family":"Neyra","sequence":"additional","affiliation":[{"name":"International Center for Infectiology Research , 69000 Lyon,","place":["France"]},{"name":"INSERM, U1111 , 69000 Lyon,","place":["France"]},{"name":"\u00c9cole Normale Sup\u00e9rieure de Lyon , 69000 Lyon,","place":["France"]},{"name":"Universit\u00e9 Lyon 1 , 69000 Lyon,","place":["France"]},{"name":"CNRS, UMR5308 , 69000 Lyon,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Laure","family":"Mathieu","sequence":"additional","affiliation":[{"name":"International Center for Infectiology Research , 69000 Lyon,","place":["France"]},{"name":"INSERM, U1111 , 69000 Lyon,","place":["France"]},{"name":"\u00c9cole Normale Sup\u00e9rieure de Lyon , 69000 Lyon,","place":["France"]},{"name":"Universit\u00e9 Lyon 1 , 69000 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