{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:09:08Z","timestamp":1777900148087,"version":"3.51.4"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-DK115668-01"],"award-info":[{"award-number":["R01-DK115668-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention Research Institute of Texas","doi-asserted-by":"publisher","award":["RP160577"],"award-info":[{"award-number":["RP160577"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic Bacteroides sp. in Itch\u2212\/\u2212 mice. Treatment with broad-spectrum antibiotics substantially reduced colonic inflammation in Itch\u2212\/\u2212 mice. Microbiota of Itch\u2212\/\u2212 mice failed to induce spontaneous colitis upon transfer to Itch+\/+ mice but aggravated chemically induced colitis. Furthermore, we found that Bacteroides vulgatus, which is expanded in Itch\u2212\/\u2212 mice, was sufficient to induce colon inflammation in Itch\u2212\/\u2212 mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1701478","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T00:25:40Z","timestamp":1584145540000},"page":"2277-2284","source":"Crossref","is-referenced-by-count":19,"title":["Gut Microbiota Contributes to Spontaneous Colitis in E3 Ligase Itch-Deficient Mice"],"prefix":"10.1093","volume":"204","author":[{"given":"Mahesh","family":"Kathania","sequence":"first","affiliation":[{"name":"Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2128-9857","authenticated-orcid":false,"given":"Elviche L","family":"Tsakem","sequence":"additional","affiliation":[{"name":"Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2862-0933","authenticated-orcid":false,"given":"Arianne L","family":"Theiss","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, School of Medicine at the Anschutz Medical Campus, University of Colorado , Aurora, CO 80045"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Venuprasad","sequence":"additional","affiliation":[{"name":"Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,4,15]]},"reference":[{"key":"2025030417511531000_r1","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1126\/science.1155725","article-title":"Evolution of mammals and their gut microbes","volume":"320","author":"Ley","year":"2008","journal-title":"Science"},{"key":"2025030417511531000_r2","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.ccell.2018.03.004","article-title":"The intestinal microbiota in colorectal cancer","volume":"33","author":"Tilg","year":"2018","journal-title":"Cancer Cell"},{"key":"2025030417511531000_r3","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.cell.2010.01.023","article-title":"Homeostasis and inflammation in the intestine","volume":"140","author":"Garrett","year":"2010","journal-title":"Cell"},{"key":"2025030417511531000_r4","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1056\/NEJMra0804647","article-title":"Inflammatory bowel disease","volume":"361","author":"Abraham","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"2025030417511531000_r5","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.chom.2014.02.005","article-title":"The treatment-naive microbiome in new-onset Crohn\u2019s disease","volume":"15","author":"Gevers","year":"2014","journal-title":"Cell Host Microbe"},{"key":"2025030417511531000_r6","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1136\/gutjnl-2012-303954","article-title":"Advances in inflammatory bowel disease pathogenesis: linking host genetics and the microbiome","volume":"62","author":"Knights","year":"2013","journal-title":"Gut"},{"key":"2025030417511531000_r7","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1146\/annurev.biochem.67.1.425","article-title":"The ubiquitin system","volume":"67","author":"Hershko","year":"1998","journal-title":"Annu. Rev. Biochem."},{"key":"2025030417511531000_r8","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/icb.2014.118","article-title":"Multifaceted role of the ubiquitin ligase Itch in immune regulation","volume":"93","author":"Venuprasad","year":"2015","journal-title":"Immunol. Cell Biol."},{"key":"2025030417511531000_r9","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.ajhg.2010.01.028","article-title":"Human ITCH E3 ubiquitin ligase deficiency causes syndromic multisystem autoimmune disease","volume":"86","author":"Lohr","year":"2010","journal-title":"Am. J. Hum. Genet."},{"key":"2025030417511531000_r10","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1038\/ni.3488","article-title":"Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-\u03b3t ubiquitination","volume":"17","author":"Kathania","year":"2016","journal-title":"Nat. Immunol."},{"key":"2025030417511531000_r11","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1073\/pnas.1000080107","article-title":"Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample","volume":"108","author":"Caporaso","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025030417511531000_r12","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1128\/AEM.03006-05","article-title":"Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB","volume":"72","author":"DeSantis","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"2025030417511531000_r13","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.cell.2004.07.002","article-title":"Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis","volume":"118","author":"Rakoff-Nahoum","year":"2004","journal-title":"Cell"},{"key":"2025030417511531000_r14","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1128\/IAI.01197-12","article-title":"Intestinal microbiota containing Barnesiella species cures vancomycin-resistant Enterococcus faecium colonization","volume":"81","author":"Ubeda","year":"2013","journal-title":"Infect. Immun."},{"key":"2025030417511531000_r15","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.ccr.2011.10.022","article-title":"The NOD-like receptor NLRP12 attenuates colon inflammation and tumorigenesis","volume":"20","author":"Zaki","year":"2011","journal-title":"Cancer Cell"},{"key":"2025030417511531000_r16","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1002\/ibd.21354","article-title":"The struggle within: microbial influences on colorectal cancer","volume":"17","author":"Arthur","year":"2011","journal-title":"Inflamm. Bowel Dis."},{"key":"2025030417511531000_r17","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/nrc3611","article-title":"Inflammation-induced cancer: crosstalk between tumours, immune cells and microorganisms","volume":"13","author":"Elinav","year":"2013","journal-title":"Nat. Rev. Cancer"},{"key":"2025030417511531000_r18","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.cell.2014.03.006","article-title":"The fire within: microbes inflame tumors","volume":"157","author":"Gagliani","year":"2014","journal-title":"Cell"},{"key":"2025030417511531000_r19","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.cell.2007.08.017","article-title":"Communicable ulcerative colitis induced by T-bet deficiency in the innate immune system","volume":"131","author":"Garrett","year":"2007","journal-title":"Cell"},{"key":"2025030417511531000_r20","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nri2295","article-title":"TH17 cells in development: an updated view of their molecular identity and genetic programming","volume":"8","author":"Dong","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"2025030417511531000_r21","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.cell.2011.04.022","article-title":"NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis","volume":"145","author":"Elinav","year":"2011","journal-title":"Cell"},{"key":"2025030417511531000_r22","doi-asserted-by":"crossref","first-page":"9862","DOI":"10.1073\/pnas.1307575110","article-title":"Microbiota-induced activation of epithelial IL-6 signaling links inflammasome-driven inflammation with transmissible cancer. [Published erratum appears in 2013 Proc. Natl. Acad. Sci. USA 110: 12852.]","volume":"110","author":"Hu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025030417511531000_r23","first-page":"700","article-title":"NOD2-mediated dysbiosis predisposes mice to transmissible colitis and colorectal cancer","volume":"123","author":"Couturier-Maillard","year":"2013","journal-title":"J. Clin. Invest."},{"key":"2025030417511531000_r24","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.chom.2011.04.009","article-title":"Commensal Bacteroides species induce colitis in host-genotype-specific fashion in a mouse model of inflammatory bowel disease","volume":"9","author":"Bloom","year":"2011","journal-title":"Cell Host Microbe"},{"key":"2025030417511531000_r25","doi-asserted-by":"crossref","first-page":"17026","DOI":"10.1038\/nmicrobiol.2017.26","article-title":"An insider\u2019s perspective: Bacteroides as a window into the microbiome","volume":"2","author":"Wexler","year":"2017","journal-title":"Nat. Microbiol."},{"key":"2025030417511531000_r26","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nature10213","article-title":"Human nutrition, the gut microbiome and the immune system","volume":"474","author":"Kau","year":"2011","journal-title":"Nature"},{"key":"2025030417511531000_r27","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.crohns.2009.11.005","article-title":"The joint-gut axis in inflammatory bowel diseases","volume":"4","author":"Brakenhoff","year":"2010","journal-title":"J. Crohn\u2019s Colitis"},{"key":"2025030417511531000_r28","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/nm.2015","article-title":"A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses","volume":"15","author":"Wu","year":"2009","journal-title":"Nat. Med."},{"key":"2025030417511531000_r29","first-page":"427","article-title":"IL-17-driven intestinal fibrosis is inhibited by Itch-mediated ubiquitination of HIC-5","volume-title":"Mucosal Immunol.","author":"Paul","year":"2018"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/204\/8\/2277\/61881922\/ji1701478.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/204\/8\/2277\/61881922\/ji1701478.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T18:42:41Z","timestamp":1741113761000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/204\/8\/2277\/7943783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,4,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1701478","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,4]]},"published":{"date-parts":[[2020,4]]}}}