{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T20:22:43Z","timestamp":1789849363174,"version":"4.0.1"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The purpose of this study was to investigate the role of pentraxin 3 (PTX3), a pivotal component of the innate immune system, in gout. Levels of PTX3 and IL-1\u03b2 in human samples were evaluated by ELISA. Development of murine gout was evaluated through the levels of cytokines (PTX3, CXCL1, and IL-1\u03b2) and neutrophil recruitment into the joint cavity. Phagocytosis of monosodium urate (MSU) crystals and caspase-1 activation were determined by flow cytometer. Acute gout patients showed elevated concentration of PTX3 in plasma and synovial fluid as compared with healthy and osteoarthritic subjects. Moreover, there was a positive correlation between intra-articular PTX3 and IL-1\u03b2 levels. PTX3 was induced in the periarticular tissue of mice postinjection of MSU crystals. Importantly, Ptx3-deficient mice showed reduced inflammation in response to MSU crystal injection compared with wild-type mice, including reduction of neutrophil recruitment into the joint cavity and IL-1\u03b2 and CXCL1 production. Interestingly, addition of PTX3 in vitro enhanced MSU crystal phagocytosis by monocytes and resulted in higher production of IL-1\u03b2 by macrophages. This contribution of PTX3 to the phagocytosis of MSU crystals and consequent production of IL-1\u03b2 occurred through a mechanism mainly dependent on Fc\u03b3RIII. Thus, our results suggest that PTX3 acts as a humoral pattern recognition molecule in gout facilitating MSU crystal phagocytosis and contributing to the pathogenesis of gouty arthritis.<\/jats:p>","DOI":"10.4049\/jimmunol.1701531","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T20:25:21Z","timestamp":1549311921000},"page":"1807-1814","source":"Crossref","is-referenced-by-count":9,"title":["The Long Pentraxin 3 Contributes to Joint Inflammation in Gout by Facilitating the Phagocytosis of Monosodium Urate Crystals"],"prefix":"10.1093","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7887-7094","authenticated-orcid":false,"given":"Nath\u00e1lia V","family":"Batista","sequence":"first","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte, Minas Gerais 31270-901 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0294-2299","authenticated-orcid":false,"given":"Marialuisa","family":"Barbagallo","sequence":"additional","affiliation":[{"name":"Humanitas Research Hospital , Rozzano 20089 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian L S","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte, Minas Gerais 31270-901 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1564-4645","authenticated-orcid":false,"given":"Thiago","family":"Castro-Gomes","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte , Minas Gerais 31270-901 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0229-6864","authenticated-orcid":false,"given":"Rene D R","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , S\u00e3o Paulo 1409-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Louzada-Junior","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , S\u00e3o Paulo 1409-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7778-569X","authenticated-orcid":false,"given":"Geraldo R C","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Discipline of Rheumatology, University of State of Rio de Janeiro , Rio de Janeiro 20550-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Humanitas University , Pieve Emanuele 20090 ,","place":["Italy"]},{"name":"The William Harvey Research Institute, Queen Mary University of London , London EC1M 6BQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro M","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte, Minas Gerais 31270-901 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-7703","authenticated-orcid":false,"given":"Cecilia","family":"Garlanda","sequence":"additional","affiliation":[{"name":"Humanitas Research Hospital , Rozzano 20089 ,","place":["Italy"]},{"name":"Humanitas University , Pieve Emanuele 20090 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-0612","authenticated-orcid":false,"given":"Fl\u00e1vio A","family":"Amaral","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte, Minas Gerais 31270-901 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"2025030417561000300_r1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/nrrheum.2015.91","article-title":"Global epidemiology of gout: prevalence, incidence and risk factors","volume":"11","author":"Kuo","year":"2015","journal-title":"Nat. Rev. Rheumatol."},{"key":"2025030417561000300_r2","first-page":"773","article-title":"Gout: excess calories, purines, and alcohol intake and beyond. Response to a urate-lowering diet","volume":"32","author":"Fam","year":"2005","journal-title":"J. Rheumatol."},{"key":"2025030417561000300_r3","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1093\/rheumatology\/keh640","article-title":"Mechanisms of inflammation in gout","volume":"44","author":"Dalbeth","year":"2005","journal-title":"Rheumatology (Oxford)"},{"key":"2025030417561000300_r4","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/nature04516","article-title":"Gout-associated uric acid crystals activate the NALP3 inflammasome","volume":"440","author":"Martinon","year":"2006","journal-title":"Nature"},{"key":"2025030417561000300_r5","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1002\/art.1780360912","article-title":"Changes in the proteins coating monosodium urate crystals during active and subsiding inflammation. 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