{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:35:59Z","timestamp":1790480159222,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Leukocyte infiltration is characteristic of lung injury and fibrosis, and its role during tissue repair and fibrosis is incompletely understood. We found that overexpression of IL-5 in transgenic mice (IL-5TG) or by adenoviral gene transfer increased bleomycin (blm)-induced lung injury, fibrosis, and eosinophilia. Surprisingly, blm-treated IL-5-deficient (IL-5\u2212\/\u2212) mice also developed pronounced pulmonary fibrosis but characterized by marked T lymphocyte infiltration and absence of eosinophilia. In both murine strains however, induction of lung TGF-\u03b2 expression was evident. Purified lung eosinophils from blm-treated IL-5TG mice stimulated \u03b1-smooth muscle actin and collagen expression in mouse lung fibroblasts, without affecting proliferation. Furthermore instillation of purified eosinophils into murine lungs resulted in extension of blm-induced lung fibrosis, thus confirming a role for eosinophils. However, lung T lymphocytes from blm-treated IL-5\u2212\/\u2212 mice were able to stimulate fibroblast proliferation but not \u03b1-smooth muscle actin or collagen expression. Blocking T cell influx by anti-CD3 Abs abrogated lung fibrosis, thus also implicating T lymphocytes as a key participant in fibrosis. Pulmonary fibrosis in IL-5TG mice was preferentially associated with type 2 cytokines (IL-4 and IL-13), whereas fibrotic lesions in IL-5\u2212\/\u2212 animals were accompanied by proinflammatory cytokine (TNF-\u03b1, IL-1\u03b2, and IFN-\u03b3) expression. We suggest that eosinophils and T cells contribute distinctly to the development of blm-induced lung fibrosis potentially via their production of different cytokine components, which ultimately induce TGF-\u03b2 expression that is intimately involved with the fibrosis.<\/jats:p>","DOI":"10.4049\/jimmunol.171.10.5470","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T18:22:42Z","timestamp":1398018162000},"page":"5470-5481","source":"Crossref","is-referenced-by-count":99,"title":["Eosinophils and T Lymphocytes Possess Distinct Roles in Bleomycin-Induced Lung Injury and Fibrosis"],"prefix":"10.1093","volume":"171","author":[{"given":"Francois","family":"Huaux","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Michigan , Ann Arbor, MI 48109-0602"},{"name":"Unit of Industrial Toxicology and Occupational Medicine, Universite\u0301 Catholique de Louvain , Brussels ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianju","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan , Ann Arbor, MI 48109-0602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget","family":"McGarry","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan , Ann Arbor, MI 48109-0602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Ullenbruch","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan , Ann Arbor, MI 48109-0602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Pathology, McMaster University , Hamilton, Ontario ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sem H","family":"Phan","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan , Ann Arbor, MI 48109-0602"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,11,15]]},"reference":[{"key":"2026020211104361800_R1","first-page":"517","volume-title":"N. 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