{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:34:02Z","timestamp":1771461242465,"version":"3.50.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infusion of donor antiviral T cells can provide protective immunity for recipients of hemopoietic progenitor cell transplants, but may cause graft-vs-host disease (GVHD). Current methods of separating antiviral T cells from the alloreactive T cells that produce GVHD are neither routine nor rapid. In a model of lethal murine CMV (MCMV) infection following MHC-mismatched bone marrow transplantation, infusion of MCMV-immune donor lymphocytes pretreated with the DNA cross-linking compound amotosalen prevented MCMV lethality without producing GVHD. Although 95% of mice receiving 30 \u00d7 106 pretreated donor lymphocytes survived beyond day +100 without MCMV disease or GVHD, all mice receiving equivalent numbers of untreated lymphocytes rapidly died of GVHD. In vitro, amotosalen blocked T cell proliferation without suppressing MCMV peptide-induced IFN-\u03b3 production by MCMV-primed CD8+ T cells. In vivo, pretreated lymphocytes reduced hepatic MCMV load by 4-log10 and promoted full hemopoietic chimerism. Amotosalen-treated, MCMV tetramer-positive memory (CD44high) CD8+ T cells persisted to day +100 following infusion, and expressed IFN-\u03b3 when presented with viral peptide. Pretreated T cells were effective at preventing MCMV lethality over a wide range of concentrations. Thus, amotosalen treatment rapidly eliminates the GVHD activity of polyclonal T cells, while preserving long-term antiviral and graft facilitation effects, and may be clinically useful for routine adoptive immunotherapy.<\/jats:p>","DOI":"10.4049\/jimmunol.171.11.6023","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:32:01Z","timestamp":1398029521000},"page":"6023-6031","source":"Crossref","is-referenced-by-count":23,"title":["Allogeneic T Cells Treated with Amotosalen Prevent Lethal Cytomegalovirus Disease without Producing Graft-versus-Host Disease Following Bone Marrow Transplantation"],"prefix":"10.1093","volume":"171","author":[{"given":"John D","family":"Roback","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, GA 30322"},{"name":"Transfusion Medicine Program, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad S","family":"Hossain","sequence":"additional","affiliation":[{"name":"Winship Cancer Institute, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levan","family":"Lezhava","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W","family":"Gorechlad","sequence":"additional","affiliation":[{"name":"Winship Cancer Institute, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabina A","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L","family":"Jaye","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Mittelstaedt","sequence":"additional","affiliation":[{"name":"Winship Cancer Institute, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohel","family":"Talib","sequence":"additional","affiliation":[{"name":"Cerus , Concord, CA 94520"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E","family":"Hearst","sequence":"additional","affiliation":[{"name":"Cerus , Concord, CA 94520"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D","family":"Hillyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, GA 30322"},{"name":"Transfusion Medicine Program, Emory University School of Medicine , Atlanta, GA 30322"},{"name":"Winship Cancer Institute, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund K","family":"Waller","sequence":"additional","affiliation":[{"name":"Winship Cancer Institute, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2003,12,1]]},"reference":[{"key":"2026020211082837500_R1","first-page":"478","volume-title":"J. 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