{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T06:21:13Z","timestamp":1783059673490,"version":"3.54.6"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\u03b3\u03b4 T lymphocytes are involved in a great variety of inflammatory and infectious responses. However, the mechanisms by which \u03b3\u03b4 T lymphocytes migrate to inflamed sites are poorly understood. In this study we investigate the role of monocyte chemotactic protein (MCP)-1 in regulating \u03b3\u03b4 T cell migration after LPS or Mycobacterium bovis bacille Calmette-Gue\u0301rin (BCG) challenge. LPS-induced \u03b3\u03b4 T cell influx was significantly inhibited by either pretreatment with dexamethasone or vaccinia virus Lister 35-kDa chemokine binding protein, vCKBP, a CC chemokine neutralizing protein, suggesting a role for CC chemokines in this phenomenon. LPS stimulation increased the expression of MCP-1 mRNA and protein at the inflammation site within 6 h. It is noteworthy that LPS was unable to increase MCP-1 production or \u03b3\u03b4 T cell recruitment in C3H\/HeJ, indicative of the involvement of Toll-like receptor 4. \u03b3\u03b4 T cells express MCP-1 receptor CCR2. Pretreatment with anti-MCP-1 mAb drastically inhibited LPS-induced in vivo \u03b3\u03b4 T cell mobilization. Indeed, MCP-1 knockout mice were unable to recruit \u03b3\u03b4 T cells to the pleural cavity after LPS stimulation, effect that could be restored by coadministration of MCP-1. In addition, BCG-induced \u03b3\u03b4 lymphocyte accumulation was significantly reduced in MCP-1 knockout mice when compared with wild-type mice. In conclusion, our results indicate that LPS-induced \u03b3\u03b4 T lymphocyte migration is dependent on Toll-like receptor 4 and sensitive to both dexamethasone and CC chemokine-binding protein inhibition. Moreover, by using MCP-1 neutralizing Abs and genetically deficient mice we show that LPS- and BCG-induced \u03b3\u03b4 T lymphocyte influx to the pleural cavity of mice is mainly orchestrated by the CC chemokine MCP-1.<\/jats:p>","DOI":"10.4049\/jimmunol.171.12.6788","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:30:32Z","timestamp":1398029432000},"page":"6788-6794","source":"Crossref","is-referenced-by-count":50,"title":["Role of Monocyte Chemotactic Protein-1\/CC Chemokine Ligand 2 on \u03b3\u03b4 T Lymphocyte Trafficking during Inflammation Induced by Lipopolysaccharide or\n                    <i>Mycobacterium bovis<\/i>\n                    Bacille Calmette-Gue\u0301rin"],"prefix":"10.1093","volume":"171","author":[{"given":"Carmen","family":"Penido","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodina\u0302mica, Instituto Oswaldo Cruz, Fundac\u0327a\u0303o Oswaldo Cruz , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Vieira-de-Abreu","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodina\u0302mica, Instituto Oswaldo Cruz, Fundac\u0327a\u0303o Oswaldo Cruz , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo T","family":"Bozza","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo C","family":"Castro-Faria-Neto","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodina\u0302mica, Instituto Oswaldo Cruz, Fundac\u0327a\u0303o Oswaldo Cruz , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patri\u0301cia T","family":"Bozza","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodina\u0302mica, Instituto Oswaldo Cruz, Fundac\u0327a\u0303o Oswaldo Cruz , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,12,15]]},"reference":[{"key":"2026020210243782200_R1","first-page":"2272","volume-title":"Proc. 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