{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:38:48Z","timestamp":1792737528265,"version":"4.5.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Adenoviral vectors can be used to deliver complex Ag to dendritic cells (DC), and thus may be ideal for stimulating broad T cell responses to viral pathogens and tumors. To test this hypothesis in a relevant primate model, we used recombinant adenovirus serotype 5 vectors expressing SIV Gag Ag to transduce monocyte-derived DC from rhesus macaques, and then immunized donor animals either by intradermal or intranodal injections. T cell responses were evaluated by ELISPOT assay using previously frozen PBMC pulsed with pools of 15-mer peptides representing the Gag sequence. Immunization resulted in rapid and potent induction of T cell responses to multiple regions of Gag, with frequencies approaching 1 Gag-specific T cell per 500 uncultured PBMC. Surprisingly, intradermal and intranodal injections generated a similar intensity and breadth of response, indicating that administration of Ag-expressing DC by either route may be equally effective at inducing immune responses. Detailed analysis of two monkeys revealed CD8+ T cell responses to several peptide epitopes of Gag not previously described, at least two of which are restricted by MHC class I alleles not currently identified. Repeated vaccination did not induce T cell responses to the adenoviral vector and did not prevent Ag-expressing DC injected under the capsule of the lymph node from migrating to the paracortex and interposing between T cells. However, boost injections of adenovirus-transduced DC were generally limited in efficacy. These findings support the use of adenovirus-transduced DC in the therapy of HIV infection and cancer.<\/jats:p>","DOI":"10.4049\/jimmunol.171.12.6875","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:30:32Z","timestamp":1398029432000},"page":"6875-6882","source":"Crossref","is-referenced-by-count":38,"title":["Adenovirus-Transduced Dendritic Cells Injected into Skin or Lymph Node Prime Potent Simian Immunodeficiency Virus-Specific T Cell Immunity in Monkeys"],"prefix":"10.1093","volume":"171","author":[{"given":"Kevin","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Alber","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Trichel","sequence":"additional","affiliation":[{"name":"Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Murphey-Corb","sequence":"additional","affiliation":[{"name":"Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C","family":"Watkins","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Gambotto","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon M","family":"Barratt-Boyes","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,12,15]]},"reference":[{"key":"2026020211102708400_R1","first-page":"245","volume-title":"Annu. Rev. Immunol.","year":"2000"},{"key":"2026020211102708400_R2","first-page":"459","volume-title":"Int. J. Cancer","year":"2001"},{"key":"2026020211102708400_R3","first-page":"365","volume-title":"J. Invest. Dermatol.","year":"2002"},{"key":"2026020211102708400_R4","first-page":"493","volume-title":"Nat. Med.","year":"2002"},{"key":"2026020211102708400_R5","first-page":"13","volume-title":"Nat. Med.","year":"2003"},{"key":"2026020211102708400_R6","first-page":"328","volume-title":"Nat. Med.","year":"1998"},{"key":"2026020211102708400_R7","first-page":"1669","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020211102708400_R8","first-page":"477","volume-title":"J. Clin. Invest.","year":"2001"},{"key":"2026020211102708400_R9","first-page":"243","volume-title":"Int. J. Cancer","year":"2001"},{"key":"2026020211102708400_R10","first-page":"1279","volume-title":"J. Exp. Med.","year":"2002"},{"key":"2026020211102708400_R11","first-page":"6952","volume-title":"Cancer Res.","year":"2002"},{"key":"2026020211102708400_R12","first-page":"27","volume-title":"Nat. Med.","year":"2003"},{"key":"2026020211102708400_R13","first-page":"102","volume-title":"Trends Immunol.","year":"2001"},{"key":"2026020211102708400_R14","first-page":"11329","volume-title":"J. Virol.","year":"2000"},{"key":"2026020211102708400_R15","first-page":"392","volume-title":"Blood","year":"1998"},{"key":"2026020211102708400_R16","first-page":"964","volume-title":"Eur. J. Immunol.","year":"1999"},{"key":"2026020211102708400_R17","first-page":"3281","volume-title":"Eur. J. Immunol.","year":"2000"},{"key":"2026020211102708400_R18","first-page":"3270","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020211102708400_R19","first-page":"1247","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020211102708400_R20","first-page":"3717","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020211102708400_R21","first-page":"2487","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020211102708400_R22","first-page":"2302","volume-title":"J. Immunol.","year":"2003"},{"key":"2026020211102708400_R23","first-page":"4543","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020211102708400_R24","first-page":"233","volume-title":"J. Exp. Med.","year":"2001"},{"key":"2026020211102708400_R25","first-page":"8179","volume-title":"J. Virol.","year":"1999"},{"key":"2026020211102708400_R26","first-page":"59","volume-title":"J. Immunol. Methods","year":"2001"},{"key":"2026020211102708400_R27","first-page":"1894","volume-title":"J. Natl. Cancer Inst.","year":"1998"},{"key":"2026020211102708400_R28","first-page":"2004","volume-title":"J. Virol.","year":"1995"},{"key":"2026020211102708400_R29","first-page":"5260","volume-title":"Cancer Res.","year":"2002"},{"key":"2026020211102708400_R30","first-page":"7968","volume-title":"J. Virol.","year":"2002"},{"key":"2026020211102708400_R31","first-page":"2859","volume-title":"Blood","year":"2002"},{"key":"2026020211102708400_R32","first-page":"10416","volume-title":"J. Virol.","year":"1999"},{"key":"2026020211102708400_R33","first-page":"R9","volume-title":"J. Clin. Invest.","year":"2000"},{"key":"2026020211102708400_R34","first-page":"6305","volume-title":"J. Virol.","year":"2003"},{"key":"2026020211102708400_R35","first-page":"149","volume-title":"Annu. Rev. Med.","year":"2002"},{"key":"2026020211102708400_R36","first-page":"5041","volume-title":"Cancer Res.","year":"2002"},{"key":"2026020211102708400_R37","first-page":"1373","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020211102708400_R38","first-page":"738","volume-title":"J. Virol.","year":"2001"},{"key":"2026020211102708400_R39","first-page":"11623","volume-title":"J. Virol.","year":"2002"},{"key":"2026020211102708400_R40","first-page":"1941","volume-title":"Gene Ther.","year":"2003"},{"key":"2026020211102708400_R41","first-page":"56","volume-title":"Cancer Res.","year":"1999"},{"key":"2026020211102708400_R42","first-page":"118","volume-title":"Cancer Immunol. Immunother.","year":"1999"},{"key":"2026020211102708400_R43","first-page":"12","volume-title":"Cancer Res.","year":"2003"},{"key":"2026020211102708400_R44","first-page":"4254","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020211102708400_R45","first-page":"1684","volume-title":"Blood","year":"2003"},{"key":"2026020211102708400_R46","first-page":"335","volume-title":"Nature","year":"2002"},{"key":"2026020211102708400_R47"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/171\/12\/6875\/62592257\/6875.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/171\/12\/6875\/62592257\/6875.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T16:10:36Z","timestamp":1770048636000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/171\/12\/6875\/8031430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":47,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2003,12,15]]},"published-print":{"date-parts":[[2003,12,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.171.12.6875","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2003,12]]},"published":{"date-parts":[[2003,12]]}}}