{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:11:01Z","timestamp":1791299461997,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Engagement of the T cell with Ag on an APC results in a series of immediate signaling events emanating from the stimulation of the TCR. These events include the induced phosphorylation of a number of cellular proteins with a subsequent increase in intracellular calcium and the restructuring of the microtubule and actin cytoskeleton within the T cell. This restructuring of the cytoskeleton culminates in the polarization of the T cell\u2019s secretory apparatus toward the engaging APC, allowing the T cell to direct secretion of cytokines toward the appropriate APC. This polarization can be monitored by analyzing the position of the microtubule-organizing center (MTOC), as it moves toward the interface of the T cell and APC. The requirements for MTOC polarization were examined at a single-cell level by studying the interaction of a Jurkat cell line expressing a fluorescently labeled MTOC with Staphylococcal enterotoxin superantigen-bound Raji B cell line, which served as the APC. We found that repolarization of the MTOC substantially followed fluxes in calcium. We also used immobilized anti-TCR mAb and Jurkat signaling mutants, defective in TCR-induced calcium increases, to determine whether signaling components that are necessary for a calcium response also play a role in MTOC polarization. We found that \u03b6-associated protein-70 as well as its substrate adaptor proteins linker for activation of T cells and Src homology 2 domain-containing leukocyte protein-76 are required for MTOC polarization. Moreover, our studies revealed that a calcium-dependent event not requiring calcineurin or calcium\/calmodulin-dependent kinase is required for TCR-induced polarization of the MTOC.<\/jats:p>","DOI":"10.4049\/jimmunol.171.2.860","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:29:26Z","timestamp":1398022166000},"page":"860-866","source":"Crossref","is-referenced-by-count":95,"title":["Linker for Activation of T Cells, \u03b6-Associated Protein-70, and Src Homology 2 Domain-Containing Leukocyte Protein-76 are Required for TCR-Induced Microtubule-Organizing Center Polarization"],"prefix":"10.1093","volume":"171","author":[{"given":"Michelle R","family":"Kuhne\u0301","sequence":"first","affiliation":[{"name":"Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"},{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"},{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Yablonski","sequence":"additional","affiliation":[{"name":"Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"},{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne N","family":"Mollenauer","sequence":"additional","affiliation":[{"name":"Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"},{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren I Richie","family":"Ehrlich","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Huppa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark M","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"},{"name":"Department of Microbiology and Immunology, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,7,15]]},"reference":[{"key":"2026020210231088100_R1","first-page":"263","volume-title":"Cell","year":"1994"},{"key":"2026020210231088100_R2","first-page":"242","volume-title":"Curr. 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