{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T19:35:51Z","timestamp":1791056151540,"version":"4.1.0"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have shown previously that Wiskott-Aldrich syndrome protein (WASP) activation at the site of T cell-APC interaction is a two-step process, with recruitment dependent on the proline-rich domain and activation dependent on binding of Cdc42-GTP to the GTPase binding domain. Here, we show that WASP recruitment occurs through binding to the C-terminal Src homology 3 domain of Nck. In contrast, WASP activation requires Vav-1. In Vav-1-deficient T cells, WASP recruitment proceeds normally, but localized activation of Cdc42 and WASP is disrupted. The recruitment and activation of WASP are coordinated by tyrosine-phosphorylated Src homology 2 domain-containing leukocyte protein of 76 kDa, which functions as a scaffold, bringing Nck and WASP into proximity with Vav-1 and Cdc42-GTP. Taken together, these findings reconstruct the signaling pathway leading from TCR ligation to localized WASP activation.<\/jats:p>","DOI":"10.4049\/jimmunol.171.3.1360","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:29:45Z","timestamp":1398029385000},"page":"1360-1368","source":"Crossref","is-referenced-by-count":138,"title":["SLP-76 Coordinates Nck-Dependent Wiskott-Aldrich Syndrome Protein Recruitment with Vav-1\/Cdc42-Dependent Wiskott-Aldrich Syndrome Protein Activation at the T Cell-APC Contact Site"],"prefix":"10.1093","volume":"171","author":[{"given":"Rong","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy L","family":"Cannon","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Chicago , Chicago, IL 60637"},{"name":"Committee on Immunology, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert T","family":"Abraham","sequence":"additional","affiliation":[{"name":"Program in Signal Transduction Research, The Burnham Institute , La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Way","sequence":"additional","affiliation":[{"name":"Cell Motility Group, Imperial Cancer Research Fund , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel D","family":"Billadeau","sequence":"additional","affiliation":[{"name":"Division of Developmental Oncology Research, Mayo Clinic , Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Bubeck-Wardenberg","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis K","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Chicago , Chicago, IL 60637"},{"name":"Committee on Immunology, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,8,1]]},"reference":[{"key":"2026020210212873400_R1","first-page":"1367","volume-title":"J. 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