{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:57:25Z","timestamp":1790006245331,"version":"4.0.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Superantigens (SAGs) aberrantly alter immune system function through simultaneous interaction with lateral surfaces of MHC class II molecules on APCs and with particular variable regions of the TCR \u03b2-chain (V\u03b2). To further define the interface between the bacterial SAG toxic shock syndrome toxin-1 (TSST-1) and the TCR, we performed alanine scanning mutagenesis within the putative TCR binding region of TSST-1 along the central \u03b1 helix adjacent to the N-terminal \u03b1 helix and the \u03b27-\u03b29 loop as well as with two universally conserved SAG residues (Leu137 and Tyr144 in TSST-1). Mutants were analyzed for multiple functional activities, and various residues appeared to play minor or insignificant roles in the TCR interaction. The locations of six residues (Gly16, Trp116, Glu132, His135, Gln136, and Gln139), each individually critical for functional activity as well as direct interaction with the human TCR V\u03b22.1-chain, indicate that the interface occurs in a novel region of the SAG molecule. Based on these data, a model of the MHC\/TSST-1\/TCR ternary complex predicts similarities seen with other characterized SAGs, although the CDR3 loop of V\u03b22.1 is probably involved in direct SAG-TCR molecular interactions, possibly contributing to the TCR V\u03b2 specificity of TSST-1.<\/jats:p>","DOI":"10.4049\/jimmunol.171.3.1385","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:29:45Z","timestamp":1398029385000},"page":"1385-1392","source":"Crossref","is-referenced-by-count":40,"title":["Functional Analysis of the TCR Binding Domain of Toxic Shock Syndrome Toxin-1 Predicts Further Diversity in MHC Class II\/Superantigen\/TCR Ternary Complexes"],"prefix":"10.1093","volume":"171","author":[{"given":"John K","family":"McCormick","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Minnesota Medical School , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J","family":"Tripp","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Minnesota Medical School , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea S","family":"Llera","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute , Rockville, MD 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J","family":"Sundberg","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute , Rockville, MD 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin M","family":"Dinges","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Minnesota Medical School , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy A","family":"Mariuzza","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute , Rockville, MD 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M","family":"Schlievert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Minnesota Medical School , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,8,1]]},"reference":[{"key":"2026020210160048200_R1","first-page":"705","volume-title":"Science","year":"1990"},{"key":"2026020210160048200_R2","first-page":"435","volume-title":"Annu. 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