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Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC\/TCR and the cell surface binding avidity of pMHC\/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC\/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal over time allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.<\/jats:p>","DOI":"10.4049\/jimmunol.171.9.4493","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:52:48Z","timestamp":1398027168000},"page":"4493-4503","source":"Crossref","is-referenced-by-count":67,"title":["Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Affinity Peptide-MHC\/TCR Interaction Overcomes Lack of CD8 Engagement"],"prefix":"10.1093","volume":"171","author":[{"given":"Samantha E","family":"Kerry","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Buslepp","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine A","family":"Cramer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Maile","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucinda L","family":"Hensley","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alma I","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Kavathas","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University , New Haven, CT 06520"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara J","family":"Vilen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"},{"name":"Department of Biochemistry and Biophysics, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey A","family":"Frelinger","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2003,11,1]]},"reference":[{"key":"2026020210195239100_R1","first-page":"82","volume-title":"Curr. 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