{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:13:29Z","timestamp":1790968409057,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>NK T cells are a unique lymphocyte population that have developmental requirements distinct from conventional T cells. Mice lacking the tyrosine kinase Fyn have 5- to 10-fold fewer mature NK T cells. This study shows that Fyn-deficient mice have decreased numbers of NK1.1\u2212 NK T cell progenitors as well. 5-Bromo-2\u2032-deoxyuridine-labeling studies indicate that the NK T cells remaining in fyn\u2212\/\u2212 mice exhibit a similar turnover rate as wild-type cells. The fyn\u2212\/\u2212 NK T cells respond to \u03b1-galactosylceramide, a ligand recognized by NK T cells, and produce cytokines, but have depressed proliferative capacity. Transgenic expression of the NK T cell-specific TCR \u03b1-chain V\u03b114J\u03b118 leads to a complete restoration of NK T cell numbers in fyn\u2212\/\u2212 mice. Together, these results suggest that Fyn may have a role before \u03b1-chain rearrangement rather than for positive selection or the peripheral upkeep of cell number. NK T cells can activate other lymphoid lineages via cytokine secretion. These secondary responses are impaired in Fyn-deficient mice, but occur normally in fyn mutants expressing the V\u03b114J\u03b118 transgene. Because this transgene restores NK T cell numbers, the lack of secondary lymphocyte activation in the fyn-mutant mice is due to the decreased numbers of NK T cells present in the mutant, rather than an intrinsic defect in the ability of the other fyn\u2212\/\u2212 lymphoid populations to respond.<\/jats:p>","DOI":"10.4049\/jimmunol.172.10.6093","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:55:35Z","timestamp":1398027335000},"page":"6093-6100","source":"Crossref","is-referenced-by-count":18,"title":["Restoration of NK T Cell Development in\n                    <i>fyn<\/i>\n                    -Mutant Mice by a TCR Reveals a Requirement for Fyn During Early NK T Cell Ontogeny"],"prefix":"10.1093","volume":"172","author":[{"given":"Paul","family":"Gadue","sequence":"first","affiliation":[{"name":"Graduate Group in Immunology, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumesh","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Feinberg School of Medicine, Northwestern University , Chicago, IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul L","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,5,15]]},"reference":[{"key":"2026020211290203200_R1","first-page":"1097","volume-title":"J. 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