{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T22:46:21Z","timestamp":1778107581959,"version":"3.51.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hemopoietic stem cells (HSC) are identified through their unique ability, at the single cell level, to long-term reconstitute all blood cell lineages. Sustained myeloid reconstitution is considered the hallmark of HSC, because myeloid progenitors and their progeny have very short half-lives. Here we demonstrate that the established practice of relying on RB6-8C5 as a myeloid specific Ab can result in overestimation of HSC frequencies because the RB6-8C5 Ab also detects Ags expressed on a sizeable population of CD3+CD8+ T cells, constitutively as well as following transplantation. Likewise, a high fraction of mice transplanted with limiting numbers of ex vivo expanded Lin\u2212Sca+kit+CD34\u2212 HSC show long-term RB6-8C5+CD3+ (lymphoid) but no RB6-8C5+CD3\u2212 (myeloid) reconstitution. Most noteworthy, the use of RB6-8C5 as a myeloid specific Ab can be deceptive by implicating the existence of lineage-restricted HSC capable of long-term reconstituting the myeloid and T, but not B, cell lineage. Because cross-lineage expression of \u201clineage-specific\u201d markers is unlikely to be unique to the blood system, claims of unexpected cell fates should be substantiated not only by acquisition of lineage-specific markers, but also absence of markers of other lineages normally derived from the investigated stem cells.<\/jats:p>","DOI":"10.4049\/jimmunol.172.3.1548","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:08:36Z","timestamp":1398028116000},"page":"1548-1552","source":"Crossref","is-referenced-by-count":14,"title":["Deceptive Multilineage Reconstitution Analysis of Mice Transplanted with Hemopoietic Stem Cells, and Implications for Assessment of Stem Cell Numbers and Lineage Potentials"],"prefix":"10.1093","volume":"172","author":[{"given":"David","family":"Bryder","sequence":"first","affiliation":[{"name":"Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ole Johan","family":"Borge","sequence":"additional","affiliation":[{"name":"Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten-Eirik W","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,2,1]]},"reference":[{"key":"2026020211171665700_R1","first-page":"393","volume-title":"Nat. Med.","year":"2001"},{"key":"2026020211171665700_R2","first-page":"575","volume-title":"Curr. Opin. Genet. Dev.","year":"2001"},{"key":"2026020211171665700_R3","first-page":"58","volume-title":"Science","year":"1988"},{"key":"2026020211171665700_R4","first-page":"242","volume-title":"Science","year":"1996"},{"key":"2026020211171665700_R5","first-page":"4781","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1990"},{"key":"2026020211171665700_R6","first-page":"1127","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020211171665700_R7","first-page":"1757","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2001"},{"key":"2026020211171665700_R8","first-page":"419","volume-title":"Blood","year":"2001"},{"key":"2026020211171665700_R9","first-page":"440","volume-title":"Exp. Hematol.","year":"1998"},{"key":"2026020211171665700_R10","first-page":"1201","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020211171665700_R11","first-page":"1006","volume-title":"Blood","year":"1995"},{"key":"2026020211171665700_R12","first-page":"1273","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020211171665700_R13","first-page":"2618","volume-title":"Blood","year":"2001"},{"key":"2026020211171665700_R14","first-page":"193","volume-title":"Immunity","year":"2000"},{"key":"2026020211171665700_R15","first-page":"3016","volume-title":"Blood","year":"2000"},{"key":"2026020211171665700_R16","first-page":"941","volume-title":"J. Exp. Med.","year":"2001"},{"key":"2026020211171665700_R17","first-page":"2012","volume-title":"Blood","year":"2000"},{"key":"2026020211171665700_R18","first-page":"1748","volume-title":"Blood","year":"2000"},{"key":"2026020211171665700_R19","first-page":"77","volume-title":"Blood","year":"1980"},{"key":"2026020211171665700_R20","first-page":"961","volume-title":"J. Clin. Invest.","year":"1998"},{"key":"2026020211171665700_R21","first-page":"675","volume-title":"Science","year":"2000"},{"key":"2026020211171665700_R22","first-page":"2399","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020211171665700_R23","first-page":"253","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020211171665700_R24","first-page":"25","volume-title":"Monoclonal Antibodies and T cell Hybridomas; Perspective and Technical Advances","year":"1981"},{"key":"2026020211171665700_R25","first-page":"201","volume-title":"Mol. Med. Today","year":"1999"},{"key":"2026020211171665700_R26","first-page":"13648","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1997"},{"key":"2026020211171665700_R27","first-page":"139","volume-title":"J. Immunol. Methods","year":"1996"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/172\/3\/1548\/62594101\/1548.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/172\/3\/1548\/62594101\/1548.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T16:17:23Z","timestamp":1770049043000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/172\/3\/1548\/8033523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,1]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2004,2,1]]},"published-print":{"date-parts":[[2004,2,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.172.3.1548","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2004,2]]},"published":{"date-parts":[[2004,2,1]]}}}