{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:53:27Z","timestamp":1790967207455,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Increased subepithelial deposition of extracellular matrix proteins is a key feature in bronchial asthma. Matrix metalloproteinase-9 (MMP-9) is a proteolytic enzyme that degrades the extracellular matrix. Tenascin is an extracellular matrix glycoprotein that is abundant in thickened asthmatic subbasement membrane. The expression of MMP-9 and tenascin reflects disease activity in asthma and airway remodeling. The molecular mechanisms regulating the expression of these proteins remain unknown. Both MMP-9 and tenascin promoters contain an Ets binding site, suggesting control by Ets-1. Thus, we hypothesized that Ets-1 expression is increased in asthma and that it contributed to enhanced MMP-9 and tenascin expression. To test this hypothesis, we determined the expression of Ets-1 in bronchial biopsies obtained from asthmatic subjects and determined the expression of Ets-1, MMP-9, and tenascin by bronchial fibroblasts activated ex vivo. We observed that nuclear extracts from TNF-\u03b1-activated fibroblasts showed increased Ets-binding activity. In addition, TNF-\u03b1-activated fibroblasts had increased expression of Ets-1 mRNA and protein, which preceded an increase in MMP-9 and tenascin mRNA. Furthermore, treatment of fibroblasts with Ets-1 antisense oligonucleotides down-regulated TNF-\u03b1-induced Ets-1, MMP-9, and, to a lesser extent, tenascin protein expression or activity. Taken together, these data demonstrate that TNF-\u03b1 increases MMP-9 and tenascin expression in bronchial fibroblasts via the transcription factor Ets-1, and suggest a role for Ets-1 in airway remodeling in asthma.<\/jats:p>","DOI":"10.4049\/jimmunol.172.3.1945","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:08:36Z","timestamp":1398028116000},"page":"1945-1952","source":"Crossref","is-referenced-by-count":54,"title":["Ets-1 Regulates TNF-\u03b1-Induced Matrix Metalloproteinase-9 and Tenascin Expression in Primary Bronchial Fibroblasts"],"prefix":"10.1093","volume":"172","author":[{"given":"Yutaka","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Medicine-Pulmonary and Critical Care Section, University of Wisconsin , Madison, WI 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phane","family":"Esnault","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Wisconsin , Madison, WI 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Wisconsin , Madison, WI 53792"},{"name":"Division of Oral Developmental Biology, Osaka University Graduate School of Dentistry , Osaka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A B","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Medicine-Pulmonary and Critical Care Section, University of Wisconsin , Madison, WI 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S","family":"Malter","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Wisconsin , Madison, WI 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nizar N","family":"Jarjour","sequence":"additional","affiliation":[{"name":"Department of Medicine-Pulmonary and Critical Care Section, University of Wisconsin , Madison, WI 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,2,1]]},"reference":[{"key":"2026020211190480100_R1","first-page":"1033","volume-title":"N. 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