{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:09:04Z","timestamp":1780445344396,"version":"3.54.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Use of the serogroup B meningococcal capsular polysaccharide (MenB CP) as a vaccine is hampered by the presence of epitopes that cross-react with human polysialic acid. As non-cross-reactive, protective capsular epitopes have also been described, we set out to develop protein mimics of one of such epitopes using as a template a highly protective mAb (mAb Seam 3) raised against a chemically modified form of the MenB CP (N-Pr MenB CP). Using phage display, anti-idiotypic single-chain Ab fragments (scFvs) were obtained from spleen cells of mice immunized with the Seam 3 mAb. Two Seam 3-specific scFvs competed with N-Pr MenB CP for binding to either mAb Seam 3 or rabbit Abs present in typing sera. Moreover, in mice and rabbits the scFvs elicited the production of Abs reacting with both N-Pr MenB CP and whole meningococci, but not with human polysialic acid. These scFv-induced Ab responses were boostable and of the Th1 type, as shown by a predominance of IgG2a. In addition, passive immunization with sera from scFv-immunized animals partially protected neonatal mice from experimental infection with group B meningococci. In conclusion, we have produced anti-idiotypic scFvs that mimic a protective MenB CP epitope and may be useful in the development of an alternative group B meningococcal vaccine.<\/jats:p>","DOI":"10.4049\/jimmunol.172.4.2461","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T23:33:57Z","timestamp":1398209637000},"page":"2461-2468","source":"Crossref","is-referenced-by-count":17,"title":["Protective Immunization against Group B Meningococci Using Anti-Idiotypic Mimics of the Capsular Polysaccharide"],"prefix":"10.1093","volume":"172","author":[{"given":"Concetta","family":"Beninati","sequence":"first","affiliation":[{"name":"Dipartimento di Patologia e Microbiologia Sperimentale, Universita\u0300 degli Studi di Messina , Messina ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Arseni","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, Universita\u0300 degli Studi di Parma , Parma ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Mancuso","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Microbiologia Sperimentale, Universita\u0300 degli Studi di Messina , Messina ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Magliani","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, Universita\u0300 degli Studi di Parma , Parma ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Conti","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, Universita\u0300 degli Studi di Parma , Parma ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Midiri","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Microbiologia Sperimentale, Universita\u0300 degli Studi di Messina , Messina ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmelo","family":"Biondo","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Microbiologia Sperimentale, Universita\u0300 degli Studi di Messina , Messina ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Polonelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, Universita\u0300 degli Studi di Parma , Parma ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Teti","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia e Microbiologia Sperimentale, Universita\u0300 degli Studi di Messina , Messina ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,2,15]]},"reference":[{"key":"2026020211174518500_R1","first-page":"147","volume-title":"Meningococcal Disease","year":"1995"},{"key":"2026020211174518500_R2","first-page":"139","volume-title":"Acta Neurol. 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