{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:00:45Z","timestamp":1791262845846,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>T regulatory cells and IL-10 have been implicated in the mechanism of immunotherapy in patients with systemic anaphylaxis following bee stings. We studied the role of IL-10 in the induction of clinical, cellular, and humoral tolerance during immunotherapy for local mucosal allergy in subjects with seasonal pollinosis. Local and systemic IL-10 responses and serum Ab concentrations were measured before\/after a double-blind trial of grass pollen (Phleum pratense, Phl P) immunotherapy. We observed local increases in IL-10 mRNA-positive cells in the nasal mucosa after 2 years of immunotherapy, but only during the pollen season. IL-10 protein-positive cells were also increased and correlated with IL-10 mRNA+ cells. These changes were not observed in placebo-treated subjects or in healthy controls. Fifteen and 35% of IL-10 mRNA signals were colocalized to CD3+ T cells and CD68+ macrophages, respectively, whereas only 1\u20132% of total CD3+ cells and 4% of macrophages expressed IL-10. Following immunotherapy, peripheral T cells cultured in the presence of grass pollen extract also produced IL-10. Immunotherapy resulted in blunting of seasonal increases in serum allergen Phl p 5-specific IgE, 60- to 80-fold increases in Phl p 5-specific IgG, and 100-fold increases in Phl p 5-specific IgG4. Post-immunotherapy serum exhibited inhibitory activity, which coeluted with IgG4, and blocked IgE-facilitated binding of allergen-IgE complexes to B cells. Both the increases in IgG and the IgG \u201cblocking\u201d activity correlated with the patients\u2019 overall assessment of improvement. Thus, grass pollen immunotherapy may induce allergen-specific, IL-10-dependent \u201cprotective\u201d IgG4 responses.<\/jats:p>","DOI":"10.4049\/jimmunol.172.5.3252","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T03:21:06Z","timestamp":1398050466000},"page":"3252-3259","source":"Crossref","is-referenced-by-count":466,"title":["Grass Pollen Immunotherapy Induces Mucosal and Peripheral IL-10 Responses and Blocking IgG Activity"],"prefix":"10.1093","volume":"172","author":[{"given":"Kayhan T","family":"Nouri-Aria","sequence":"first","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra A","family":"Wachholz","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N","family":"Francis","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikila R","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha M","family":"Walker","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa K","family":"Wilcock","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven Q","family":"Staple","sequence":"additional","affiliation":[{"name":"Central Laboratory of Netherlands, Netherlands Red Cross, Blood Transfusion Service , Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C","family":"Aalberse","sequence":"additional","affiliation":[{"name":"Central Laboratory of Netherlands, Netherlands Red Cross, Blood Transfusion Service , Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J","family":"Till","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R","family":"Durham","sequence":"additional","affiliation":[{"name":"Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,3,1]]},"reference":[{"key":"2025032707434853400_R1","first-page":"461","volume-title":"Allergy","year":"1998"},{"key":"2025032707434853400_R2","first-page":"558","volume-title":"J. 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