{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:51:24Z","timestamp":1761630684358,"version":"3.40.3"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Removal of alloreactive cells by either thymic deletion or deletion\/anergy in the periphery is regarded as crucial to the development of tolerance. Dyes, such as CFSE, that allow monitoring of cell division suggest that in vitro proliferation could be a used as a way of \u201cpruning\u201d alloreactive cells while retaining a normal immune repertoire with retention of memory to previously encountered pathogens. This would overcome the problems occurring as a result of therapies that use massive depletion of T cells to allow acceptance of organ transplants or bone marrow grafts. We therefore used a skin graft model of CD4-mediated T cell rejection across a major H-2 mismatch (C57BL\/6 (H-2b) to BALB\/c (H-2d) mice) to evaluate whether nondividing CD4+ T cells derived from a mixed lymphocyte culture would exhibit tolerance to a skin graft from the initial stimulator strain. We demonstrate that selective removal of dividing alloreactive CD4+ T cells resulted in marked specific prolongation of allogeneic skin graft survival, and that the nondividing CD4+ T cells retained a broad TCR repertoire and the ability to maintain memory. This novel way of depleting alloreactive T cells may serve as a useful strategy in combination with other mechanisms to achieve transplant tolerance.<\/jats:p>","DOI":"10.4049\/jimmunol.173.11.6574","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T04:54:49Z","timestamp":1398142489000},"page":"6574-6582","source":"Crossref","is-referenced-by-count":12,"title":["\u201cPruning\u201d of Alloreactive CD4+ T Cells Using 5- (and 6-)Carboxyfluorescein Diacetate Succinimidyl Ester Prolongs Skin Allograft Survival"],"prefix":"10.1093","volume":"173","author":[{"given":"Debbie","family":"Watson","sequence":"first","affiliation":[{"name":"Center for Kidney Research, The Children\u2019s Hospital at Westmead , Westmead, New South Wales ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoff Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Kidney Research, The Children\u2019s Hospital at Westmead , Westmead, New South Wales ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Sartor","sequence":"additional","affiliation":[{"name":"Westmead Institute of Cancer Research, Westmead Hospital , Westmead, New South Wales ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen I","family":"Alexander","sequence":"additional","affiliation":[{"name":"Center for Kidney Research, The Children\u2019s Hospital at Westmead , Westmead, New South Wales ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,12,1]]},"reference":[{"first-page":"2013","volume-title":"J. 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