{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T15:06:08Z","timestamp":1770995168551,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It has been known for some time that functional properties of dendritic cells (DC), and in particular their ability to process and present Ags to T cells, can be modulated by cytokine-induced maturation and by interactions with tumor cells. However, the molecular basis for these functional changes is unknown. We have investigated whether changes in expression of Ag-processing machinery (APM) components in DC are associated with alterations in their ability to present tumor-derived Ags to T cells. Using a panel of mAbs specific for individual APM components and a quantitative flow cytometry-based method, the level of APM components was measured in DC generated from peripheral blood monocytes of 12 normal donors and of 8 patients with cancer. Immature DC had significantly lower (p &amp;lt; 0.01) expression of MB1, LMP-7, LMP-10, TAP-1, and tapasin than mature DC. However, maturation in the presence of a cytokine mixture up-regulated expression of these components in DC obtained from normal donors and patients with cancer. Immature DC incubated with tumor cells had significantly lower (p &amp;lt; 0.001) expression of MB1, LMP-2, LMP-7, LMP-10, and endoplasmic reticulum p75 than controls. These changes were associated with a decreased ability of DC to present tumor-derived Ags to T cells, as measured in ELISPOT assays and with apoptosis of T cells in DC-T cell cultures. Thus, tumor cells have a significant suppressive effect on DC; however, ex vivo maturation of DC derived from patients with cancer in a polarizing cytokine mix restores normal expression of APM components and Ag-processing capabilities.<\/jats:p>","DOI":"10.4049\/jimmunol.173.3.1526","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T23:38:25Z","timestamp":1398209905000},"page":"1526-1534","source":"Crossref","is-referenced-by-count":72,"title":["Antigen-Processing Machinery in Human Dendritic Cells: Up-Regulation by Maturation and Down-Regulation by Tumor Cells"],"prefix":"10.1093","volume":"173","author":[{"given":"Theresa L","family":"Whiteside","sequence":"first","affiliation":[{"name":"University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine , Pittsburgh, PA"},{"name":"Departments of Pathology, Immunology and Otolaryngology, University of Pittsburgh School of Medicine , Pittsburgh, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Stanson","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine , Pittsburgh, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R","family":"Shurin","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine , Pittsburgh, PA"},{"name":"Departments of Pathology, Immunology and Otolaryngology, University of Pittsburgh School of Medicine , Pittsburgh, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soldano","family":"Ferrone","sequence":"additional","affiliation":[{"name":"Roswell Park Cancer Institute and Department of Immunology , Buffalo, NY 14263"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,8,1]]},"reference":[{"key":"2026020212070568100_R1","first-page":"245","volume-title":"Nature","year":"1998"},{"key":"2026020212070568100_R2","first-page":"859","volume-title":"Exp. 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