{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:29:34Z","timestamp":1789187374264,"version":"build-2803163510"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infection of mice with the nematode parasite Trichinella spiralis induces changes in the proteome of the jejunal epithelium, including substantial up-regulation of a novel variant of interlectin. In this study we sequence this novel lectin, termed intelectin-2, and compare expression levels during T. spiralis infection of resistant (BALB\/c) with susceptible (C57BL\/10) mouse strains. Intelectin-2 was cloned and sequenced from BALB\/c mRNA extracted on day 14 of infection, and was found to have 91% amino acid identity with intelectin (within our study termed intelectin-1). Intelectin-2 transcripts were up-regulated early (day 3) during infection with T. spiralis in BALB\/c mice, suggesting an innate response, and levels remained high through to day 14 (time of parasite rejection). Immunohistochemistry of jejunal sections with a rabbit polyclonal Ab to Xenopus laevis 35-kDa cortical granule lectin (XL35; 68% identity with intelectin-2) followed a similar pattern, with intense labeling of goblet and Paneth cells at day 14. However, intelectin-2 transcripts and protein were absent, and immunohistochemistry negative when C57BL\/10 mice were infected with T. spiralis. Genomic PCR and Southern blotting confirmed that the intelectin-2 gene is absent from the C57BL\/10 genome. The presence of intelectin-2 in resistant BALB\/c mice, its absence from the susceptible C57BL\/10 strain and the kinetics of its up-regulation during T. spiralis infection suggest that this novel lectin may serve a protective role in the innate immune response to parasite infection.<\/jats:p>","DOI":"10.4049\/jimmunol.173.3.1894","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T23:38:25Z","timestamp":1398209905000},"page":"1894-1901","source":"Crossref","is-referenced-by-count":107,"title":["Innate BALB\/c Enteric Epithelial Responses to\n                    <i>Trichinella spiralis<\/i>\n                    : Inducible Expression of a Novel Goblet Cell Lectin, Intelectin-2, and Its Natural Deletion in C57BL\/10 Mice"],"prefix":"10.1093","volume":"173","author":[{"given":"Alan D","family":"Pemberton","sequence":"first","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela A","family":"Knight","sequence":"additional","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Gamble","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Cambridge , Cambridge ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H","family":"Colledge","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Cambridge , Cambridge ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Kyu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Georgia , Athens, GA 30602-7229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Pierce","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Georgia , Athens, GA 30602-7229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh R P","family":"Miller","sequence":"additional","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,8,1]]},"reference":[{"key":"2026020212084927800_R1","first-page":"1280","volume-title":"FASEB J.","year":"1996"},{"key":"2026020212084927800_R2","first-page":"553","volume-title":"Gut","year":"1975"},{"key":"2026020212084927800_R3","first-page":"516","volume-title":"Gastroenterology","year":"1995"},{"key":"2026020212084927800_R4","first-page":"275","volume-title":"J. Histochem. Cytochem.","year":"1997"},{"key":"2026020212084927800_R5","first-page":"S77","volume-title":"Parasitology","year":"1987"},{"key":"2026020212084927800_R6","first-page":"32106","volume-title":"J. Biol. Chem.","year":"2000"},{"key":"2026020212084927800_R7","first-page":"1101","volume-title":"Proteomics","year":"2004"},{"key":"2026020212084927800_R8","first-page":"759","volume-title":"Biochem. Biophys. Res. Commun.","year":"1998"},{"key":"2026020212084927800_R9","first-page":"7520","volume-title":"J. Biol. Chem.","year":"1982"},{"key":"2026020212084927800_R10","first-page":"367","volume-title":"Glycobiology","year":"1997"},{"key":"2026020212084927800_R11","first-page":"368","volume-title":"Dev. Genes Evol.","year":"2003"},{"key":"2026020212084927800_R12","first-page":"23456","volume-title":"J. Biol. Chem.","year":"2001"},{"key":"2026020212084927800_R13","first-page":"65","volume-title":"Glycobiology","year":"2001"},{"key":"2026020212084927800_R14","first-page":"15771","volume-title":"Biochemistry","year":"2001"},{"key":"2026020212084927800_R15","first-page":"675","volume-title":"Parasite Immunol.","year":"1990"},{"key":"2026020212084927800_R16","first-page":"215","volume-title":"Parasitology","year":"1977"},{"key":"2026020212084927800_R17","first-page":"1849","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020212084927800_R18","first-page":"565","volume-title":"Anat. Rec.","year":"1981"},{"key":"2026020212084927800_R19","first-page":"67","volume-title":"Parasite Immunol.","year":"2002"},{"key":"2026020212084927800_R20","first-page":"491","volume-title":"Am. J. Pathol.","year":"1998"},{"key":"2026020212084927800_R21","first-page":"308","volume-title":"Immunology","year":"1997"},{"key":"2026020212084927800_R22","first-page":"2672","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020212084927800_R23","first-page":"117","volume-title":"Parasitology","year":"1992"},{"key":"2026020212084927800_R24","first-page":"878","volume-title":"Genome Res.","year":"1999"},{"key":"2026020212084927800_R25","first-page":"1024","volume-title":"Cytokine","year":"2000"},{"key":"2026020212084927800_R26","first-page":"13035","volume-title":"J. Am. Chem. Soc.","year":"2002"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/173\/3\/1894\/62609591\/1894.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/173\/3\/1894\/62609591\/1894.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:09:50Z","timestamp":1770052190000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/173\/3\/1894\/8035420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8,1]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2004,8,1]]},"published-print":{"date-parts":[[2004,8,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.173.3.1894","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2004,8]]},"published":{"date-parts":[[2004,8,1]]}}}