{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:28:17Z","timestamp":1761863297702,"version":"3.40.3"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Intestinal autoimmune diseases are thought to be associated with a breakdown in tolerance, leading to mucosal lymphocyte activation perhaps as a result of encounter with bacterium-derived Ag. To study mucosal CD8+ T cell activation, tolerance, and polarization of autoimmune reactivity to self-Ag, we developed a novel (Fabpl4\u00d7 at \u2212132-OVA) transgenic mouse model expressing a truncated form of OVA in intestinal epithelia of the terminal ileum and colon. We found that OVA-specific CD8+ T cells were partially tolerant to intestinal epithelium-derived OVA, because oral infection with Listeria monocytogenes-encoding OVA did not elicit an endogenous OVA-specific MHC class I tetramer+CD8+ T cell response and IFN-\u03b3-, IL-4-, and IL-5-secreting T cells were decreased in the Peyer\u2019s patches, mesenteric lymph nodes, and intestinal mucosa of transgenic mice. Adoptive transfer of OVA-specific CD8+ (OT-I) T cells resulted in their preferential expansion in the Peyer\u2019s patches and mesenteric lymph nodes and subsequently in the epithelia and lamina propria but failed to cause mucosal inflammation. Thus, CFSE-labeled OT-I cells greatly proliferated in these tissues by 5 days posttransfer. Strikingly, OT-I cell-transferred Fabpl4\u00d7 at \u2212132-OVA transgenic mice underwent a transient weight loss and developed a CD8+ T cell-mediated acute enterocolitis 5 days after oral L. monocytogenes-encoding OVA infection. These findings indicate that intestinal epithelium-derived \u201cself-Ag\u201d gains access to the mucosal immune system, leading to Ag-specific T cell activation and clonal deletion. However, when Ag is presented in the context of bacterial infection, the associated inflammatory signals drive Ag-specific CD8+ T cells to mediate intestinal immunopathology.<\/jats:p>","DOI":"10.4049\/jimmunol.173.7.4324","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T02:56:23Z","timestamp":1398048983000},"page":"4324-4330","source":"Crossref","is-referenced-by-count":30,"title":["Intestinal Epithelial Antigen Induces Mucosal CD8 T Cell Tolerance, Activation, and Inflammatory Response"],"prefix":"10.1093","volume":"173","author":[{"given":"Zhanju","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Immunology, University of Connecticut Health Center , Farmington, CT 06030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Lefranc\u0327ois","sequence":"additional","affiliation":[{"name":"Division of Immunology, University of Connecticut Health Center , Farmington, CT 06030"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,10,1]]},"reference":[{"first-page":"1004","volume-title":"Nat. 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