{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:33:18Z","timestamp":1791286398292,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The leukocyte-specific integrin, LFA-1, plays a critical role in trafficking of T cells to both lymphoid and nonlymphoid tissues. However, the role of LFA-1 in T cell activation in vivo has been less well understood. Although there have been reports describing LFA-1-deficient T cell response defects in vivo, due to impaired migration to lymphoid structures and to sites of effector function in the absence of LFA-1, it has been difficult to assess whether T cells also have a specific activation defect in vivo. We examined the role of LFA-1 in CD4+ T cell activation in vivo by using a system that allows for segregation of the migration and activation defects through the adoptive transfer of LFA-1-deficient (CD18\u2212\/\u2212) CD4+ T cells from DO11.10 Ag-specific TCR transgenic mice into wild-type BALB\/c mice. We find that in addition to its role in trafficking to peripheral lymph nodes, LFA-1 is required for optimal CD4+ T cell priming in vivo upon s.c. immunization. CD18\u2212\/\u2212 DO11.10 CD4+ T cells primed in the lymph nodes demonstrate defects in IL-2 and IFN-\u03b3 production. In addition, recipient mice adoptively transferred with CD18\u2212\/\u2212 DO11.10 CD4+ T cells demonstrate a defect in OVA-specific IgG2a production after s.c. immunization. The defect in priming of CD18\u2212\/\u2212 CD4+ T cells persists even in the presence of proliferating CD18+\/\u2212 CD4+ T cells and in lymphoid structures to which there is no migration defect. Taken together, these results demonstrate that LFA-1 is required for optimal CD4+ T cell priming in vivo.<\/jats:p>","DOI":"10.4049\/jimmunol.173.7.4443","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T02:56:23Z","timestamp":1398048983000},"page":"4443-4451","source":"Crossref","is-referenced-by-count":79,"title":["LFA-1 on CD4+ T Cells Is Required for Optimal Antigen-Dependent Activation In Vivo"],"prefix":"10.1093","volume":"173","author":[{"given":"Sravanthi","family":"Kandula","sequence":"first","affiliation":[{"name":"Department of Medicine, Section of Gastroenterology, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Gastroenterology, University of Chicago , Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,10,1]]},"reference":[{"key":"2025032617341213000_R1","first-page":"596","volume-title":"J. 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