{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:13:48Z","timestamp":1790864028113,"version":"4.1.0"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The molecular changes that restrict multipotent murine thymocytes to the T cell lineage and render them responsive to Ag receptor signals remain poorly understood. In this study, we report our analysis of the role of the Ets transcription factor, Spi-B, in this process. Spi-B expression is acutely induced coincident with T cell lineage commitment at the CD4\u2212CD8\u2212CD44\u2212CD25+ (DN3) stage of thymocyte development and is then down-regulated as thymocytes respond to pre-TCR signals and develop beyond the \u03b2-selection checkpoint to the CD4\u2212CD8\u2212CD44\u2212CD25\u2212 (DN4) stage. We found that dysregulation of Spi-B expression in DN3 thymocytes resulted in a dose-dependent perturbation of thymocyte development. Indeed, DN3 thymocytes expressing approximately five times the endogenous level of Spi-B were arrested at the \u03b2-selection checkpoint, due to impaired induction of Egr proteins, which are important molecular effectors of the \u03b2-selection checkpoint. T lineage-committed DN3 thymocytes expressing even higher levels of Spi-B were diverted to the dendritic cell lineage. Thus, we demonstrate that the prescribed modulation of Spi-B expression is important for T lineage commitment and differentiation beyond the \u03b2-selection checkpoint; and we provide insight into the mechanism underlying perturbation of development when that expression pattern is disrupted.<\/jats:p>","DOI":"10.4049\/jimmunol.174.10.6184","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T04:56:19Z","timestamp":1398142579000},"page":"6184-6194","source":"Crossref","is-referenced-by-count":54,"title":["Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks \u03b2-Selection"],"prefix":"10.1093","volume":"174","author":[{"given":"Juliette M","family":"Lefebvre","sequence":"first","affiliation":[{"name":"Immunobiology Working Group, Division of Basic Sciences, Fox Chase Cancer Center , Philadelphia, PA 19111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u0308lle C","family":"Haks","sequence":"additional","affiliation":[{"name":"Immunobiology Working Group, Division of Basic Sciences, Fox Chase Cancer Center , Philadelphia, PA 19111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael O","family":"Carleton","sequence":"additional","affiliation":[{"name":"Immunobiology Working Group, Division of Basic Sciences, Fox Chase Cancer Center , Philadelphia, PA 19111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Rhodes","sequence":"additional","affiliation":[{"name":"Immunobiology Working Group, Division of Basic Sciences, Fox Chase Cancer Center , Philadelphia, PA 19111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gomathinayagam","family":"Sinnathamby","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University , Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Celeste","family":"Simon","sequence":"additional","affiliation":[{"name":"Abramson Family Cancer Research Institute, Howard Hughes Medical Institute, University of Pennsylvania School of Medicine , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence C","family":"Eisenlohr","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University , Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Ann","family":"Garrett-Sinha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, State University of New York , Buffalo, NY 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L","family":"Wiest","sequence":"additional","affiliation":[{"name":"Immunobiology Working Group, Division of Basic Sciences, Fox Chase Cancer Center , Philadelphia, PA 19111"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,5,15]]},"reference":[{"key":"2025032706470262300_R1","first-page":"39","volume-title":"Immunol. 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