{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:06:50Z","timestamp":1790060810589,"version":"4.0.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Intracellular cAMP may inhibit T cell activation and proliferation via activation of the cAMP-dependent protein kinase, PKA. PKA signaling is maintained through interactions of the regulatory subunit with A-kinase anchoring proteins (AKAPs). We demonstrated that T cells contain AKAPs and now ask whether PKA anchoring to AKAPs via the RII\u03b1 regulatory subunit is necessary for cAMP-mediated inhibition of T cell activation. We studied the immune systems of mice lacking the RII\u03b1 regulatory subunit of PKA (\u2212\/\u2212) and the ability of cells isolated from these mice to respond to cAMP. Dissection of spleen and thymus from wild-type (WT) and \u2212\/\u2212 mice, single cell suspensions generated from these organs, and flow cytometry analysis illustrate that the gross morphology, cell numbers, and cell populations in the spleen and thymus of the \u2212\/\u2212 mice are similar to WT controls. In vitro, splenocytes from \u2212\/\u2212 mice respond to anti-CD3\/anti-CD28 and PMA\/ionomycin stimulation and produce IL-2 similar to WT. Cytokine analysis revealed no significant difference in Th1 or Th2 differentiation. Finally, equivalent frequencies of CD8+ IFN-\u03b3 producing effector cells were stimulated upon infection of WT or \u2212\/\u2212 mice with Listeria monocytogenes. These data represent the first study of the role of RII\u03b1 in the immune system in vivo and provide evidence that T cell development, homeostasis, and the generation of a cell-mediated immune response are not altered in the RII\u03b1 \u2212\/\u2212 mice, suggesting either that RII\u03b1 is not required for normal immune function or that other proteins are able to compensate for RII\u03b1 function.<\/jats:p>","DOI":"10.4049\/jimmunol.174.11.6847","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T01:21:28Z","timestamp":1398129688000},"page":"6847-6853","source":"Crossref","is-referenced-by-count":9,"title":["The Role of Protein Kinase A Anchoring via the RII\u03b1 Regulatory Subunit in the Murine Immune System"],"prefix":"10.1093","volume":"174","author":[{"given":"Robynn V","family":"Schillace","sequence":"first","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah F","family":"Andrews","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah G","family":"Galligan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly A","family":"Burton","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly J","family":"Starks","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"},{"name":"Earle A. Chiles Research Institute, Providence Medical Center , Portland, OR 97213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H G Archie","family":"Bouwer","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"},{"name":"Earle A. Chiles Research Institute, Providence Medical Center , Portland, OR 97213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G Stanley","family":"McKnight","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P","family":"Davey","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W","family":"Carr","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"},{"name":"Department of Endocrinology, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,6,1]]},"reference":[{"key":"2026020213054468400_R1","first-page":"5255","volume-title":"J. 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