{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:25:19Z","timestamp":1791289519014,"version":"4.3.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We previously demonstrated that L-selectin (CD62L)-dependent T cell homing to lymph nodes (LN) is required for tolerance induction to alloantigen. To explore the mechanisms of this observation, we analyzed the development and distribution of regulatory T cells (Treg), which play an important protective role against allograft rejection in transplantation tolerance. Alloantigen-specific tolerance was induced using either anti-CD2 plus anti-CD3 mAbs, or anti-CD40L mAbs plus donor-specific transfusion, in fully mismatched (BALB\/c donor, C57BL\/6 recipient) vascularized cardiac allografts. An expansion of CD4+CD25+CD62Lhigh T cells was observed specifically within the LN of tolerant animals, but not in other anatomic sites or under nontolerizing conditions. These cells exhibited a substantial up-regulation of Foxp3 expression as measured by real-time PCR and by fluorescent immunohistochemistry, and possessed alloantigen-specific suppressor activity. Neither LN nor other lymphoid cells expressed the regulatory phenotype if recipients were treated with anti-CD62L mAbs, which both prevented LN homing and caused early allograft rejection. However, administration of FTY720, a sphingosine 1-phosphate receptor modulator that induces CD62L-independent T cell accumulation in the LNs, restored CD4+CD25+ Treg in the LNs along with graft survival. These data suggest that alloantigen-specific Foxp3+CD4+CD25+ Treg develop and are required within the LNs during tolerization, and provide compelling evidence that distinct lymphoid compartments play critical roles in transplantation tolerance.<\/jats:p>","DOI":"10.4049\/jimmunol.174.11.6993","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T01:21:28Z","timestamp":1398129688000},"page":"6993-7005","source":"Crossref","is-referenced-by-count":150,"title":["Lymph Node Occupancy Is Required for the Peripheral Development of Alloantigen-Specific\n                    <i>Foxp3<\/i>\n                    + Regulatory T Cells"],"prefix":"10.1093","volume":"174","author":[{"given":"Jordi C","family":"Ochando","sequence":"first","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam C","family":"Yopp","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Garin","sequence":"additional","affiliation":[{"name":"Immunobiology Center, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yansui","family":"Li","sequence":"additional","affiliation":[{"name":"Recanati\/Miller Transplantation Institute, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Boros","sequence":"additional","affiliation":[{"name":"Recanati\/Miller Transplantation Institute, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Llodra","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaozhong","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"},{"name":"Recanati\/Miller Transplantation Institute, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio A","family":"Lira","sequence":"additional","affiliation":[{"name":"Immunobiology Center, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy R","family":"Krieger","sequence":"additional","affiliation":[{"name":"Recanati\/Miller Transplantation Institute, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S","family":"Bromberg","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Mount Sinai School of Medicine , New York, NY 10029"},{"name":"Recanati\/Miller Transplantation Institute, Mount Sinai School of Medicine , New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,6,1]]},"reference":[{"key":"2026020213053986000_R1","first-page":"548","volume-title":"Nature","year":"1984"},{"key":"2026020213053986000_R2","first-page":"1125","volume-title":"Science","year":"1992"},{"key":"2026020213053986000_R3","first-page":"7249","volume-title":"J. 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