{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:34:04Z","timestamp":1791239644047,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DNA polymerase (pol) \u03b7 participates in hypermutation of A:T bases in Ig genes because humans deficient for the polymerase have fewer substitutions of these bases. To determine whether polymerase \u03b7 is also responsible for the well-known preference for mutations of A vs T on the nontranscribed strand, we sequenced variable regions from three patients with xeroderma pigmentosum variant (XP-V) disease, who lack polymerase \u03b7. The frequency of mutations in the intronic region downstream of rearranged JH4 gene segments was similar between XP-V and control clones; however, there were fewer mutations of A:T bases and correspondingly more substitutions of C:G bases in the XP-V clones (p &amp;lt; 10\u22127). There was significantly less of a bias for mutations of A compared with T nucleotides in the XP-V clones compared with control clones, whereas the frequencies for mutations of C and G were identical in both groups. An analysis of mutations in the WA sequence motif suggests that polymerase \u03b7 generates more mutations of A than T on the nontranscribed strand. This in vivo data from polymerase \u03b7-deficient B cells correlates well with the in vitro specificity of the enzyme. Because polymerase \u03b7 inserts more mutations opposite template T than template A, it would generate more substitutions of A on the newly synthesized strand.<\/jats:p>","DOI":"10.4049\/jimmunol.174.12.7781","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T18:24:02Z","timestamp":1398018242000},"page":"7781-7786","source":"Crossref","is-referenced-by-count":65,"title":["DNA Polymerase \u03b7 Contributes to Strand Bias of Mutations of A versus T in Immunoglobulin Genes"],"prefix":"10.1093","volume":"174","author":[{"given":"Vladimir I","family":"Mayorov","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences, Mercer University School of Medicine , Macon, GA 31207"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor B","family":"Rogozin","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health , Bethesda, MD 20894"},{"name":"Institute of Cytology and Genetics , Novosibirsk ,","place":["Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda R","family":"Adkison","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Mercer University School of Medicine , Macon, GA 31207"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia J","family":"Gearhart","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health , Baltimore, MD 21224"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,6,15]]},"reference":[{"key":"2026020213034420900_R1","first-page":"553","volume-title":"Cell","year":"2000"},{"key":"2026020213034420900_R2","first-page":"4102","volume-title":"Proc. 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