{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:21:43Z","timestamp":1791159703641,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for the treatment of inflammatory diseases and target cyclooxygenases 1 and 2 (Cox-1, Cox-2) that are responsible for PG production. Newer Cox-2-selective drugs have been heavily prescribed to quench inflammation. Little is known about whether or not these drugs influence human B lymphocytes and their ability to produce Ab. We report herein that activated human B cells not only highly express Cox-2 and produce PGs, but that the NSAID indomethacin and Cox-2-selective drugs profoundly inhibit the ability of human B cells to produce IgG and IgM in vitro. Human blood B cells highly express Cox-2 mRNA and protein and produce PGs after activation with CD40L, pansorbin, or CD40L plus BCR engagement. Cox-2 is also highly expressed by human tonsil B cells, as shown by immunohistochemistry. Cox-inhibiting drugs modestly affect purified B cell proliferation but profoundly reduce Ab production. The ability of whole blood to produce IgM and IgG following stimulation is also strongly inhibited. In support that Cox-2 plays a seminal role in B lymphocyte Ab production, Cox-2 knockout mice have 64% less IgM and 35% less IgG than normal littermate controls. These findings support that NSAIDs and the new Cox-2-selective drugs have an unsuspected target, the B cell, and attenuate Ab production in humans. Use of NSAIDs may therefore influence autoantibody production in autoimmune diseases and may dampen humoral immunity in response to antigenic challenge\/vaccination.<\/jats:p>","DOI":"10.4049\/jimmunol.174.5.2619","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:48:17Z","timestamp":1398023297000},"page":"2619-2626","source":"Crossref","is-referenced-by-count":82,"title":["Activated Human B Lymphocytes Express Cyclooxygenase-2 and Cyclooxygenase Inhibitors Attenuate Antibody Production"],"prefix":"10.1093","volume":"174","author":[{"given":"Elizabeth P","family":"Ryan","sequence":"first","affiliation":[{"name":"Department of Environmental Medicine, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J","family":"Pollack","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas I","family":"Murant","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven H","family":"Bernstein","sequence":"additional","affiliation":[{"name":"The Lymphoma Biology Program of the James P. Wilmot Cancer Center , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond E","family":"Felgar","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Rochester Medical Center , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P","family":"Phipps","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry , Rochester, NY 14642"},{"name":"The Lymphoma Biology Program of the James P. Wilmot Cancer Center , Rochester, NY 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,3,1]]},"reference":[{"key":"2026020214094869700_R1","first-page":"144","volume-title":"Trends Immunol.","year":"2002"},{"key":"2026020214094869700_R2","first-page":"69","volume-title":"Int. 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