{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:11:03Z","timestamp":1791180663874,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Telomerase reverse transcriptase (hTERT) represents an attractive target for cancer immunotherapy because hTERT is reactivated in most human tumors. A clinical trial was initiated in which hTERT mRNA-transfected dendritic cells (DC) were administered to 20 patients with metastatic prostate cancer. Nine of these subjects received DC transfected with mRNA encoding a chimeric lysosome-associated membrane protein-1 (LAMP) hTERT protein, allowing for concomitant induction of hTERT-specific CD8+ and CD4+ T cell responses. Treatment was well tolerated. Intense infiltrates of hTERT-specific T cells were noted at intradermal injection sites after repeated vaccination. In 19 of 20 subjects, expansion of hTERT-specific CD8+ T cells was measured in the peripheral blood of study subjects, with 0.9\u20131.8% of CD8+ T cells exhibiting Ag specificity. Patients immunized with the chimeric LAMP hTERT vaccine developed significantly higher frequencies of hTERT-specific CD4+ T cells than subjects receiving DC transfected with the unmodified hTERT template. Moreover, CTL-mediated killing of hTERT targets was enhanced in the LAMP hTERT group, suggesting that an improved CD4+ response could augment a CTL response. Vaccination was further associated with a reduction of prostate-specific Ag velocity and molecular clearance of circulating micrometastases. Our findings provide a rationale for further development of hTERT-transfected DC vaccines in the treatment of prostate and other cancers.<\/jats:p>","DOI":"10.4049\/jimmunol.174.6.3798","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:13:50Z","timestamp":1398118430000},"page":"3798-3807","source":"Crossref","is-referenced-by-count":303,"title":["Telomerase mRNA-Transfected Dendritic Cells Stimulate Antigen-Specific CD8+ and CD4+ T Cell Responses in Patients with Metastatic Prostate Cancer"],"prefix":"10.1093","volume":"174","author":[{"given":"Zhen","family":"Su","sequence":"first","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Dannull","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin K","family":"Yang","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Dahm","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Coleman","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Yancey","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Sichi","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Niedzwiecki","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Boczkowski","sequence":"additional","affiliation":[{"name":"Department of Surgery, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli","family":"Gilboa","sequence":"additional","affiliation":[{"name":"Department of Surgery, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Vieweg","sequence":"additional","affiliation":[{"name":"Genitourinary Cancer Immunotherapy Program, Division of Urology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,3,15]]},"reference":[{"key":"2026020212271610900_R1","first-page":"787","volume-title":"Eur. 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