{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:55:49Z","timestamp":1772160949574,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although the pathogenesis of collagen-induced arthritis (CIA), a model of rheumatoid arthritis, is mediated by both collagen-specific CD4+ T cells and Ab specific for type II collagen (CII), the role of CII-specific T cells in the pathogenesis of CIA remains unclear. Using tetrameric HLA-DR1 with a covalently bound immunodominant CII peptide, CII259\u2013273, we studied the development of the CII-specific T cell response in the periphery and arthritic joints of DR1 transgenic mice. Although the maximum number of DR1-CII-tetramer+ cells was detected in draining lymph nodes 10 days postimmunization, these T cells accounted for only 1% or less of the CD4+ population. After day 10, their numbers gradually decreased, but were still detectable on day 130. Examination of TCR expression and changes in CD62L, CD44high, and CD69 expression by these T cells indicated that they expressed a limited TCR-BV repertoire and had clearly undergone activation. RT-PCR analysis of cytokine expression by the tetramer+ T cells compared with tetramer\u2212 cells indicated the tetramer+ cells expressed high levels of Th1 and proinflammatory cytokines, including IL-2, IFN-\u03b3, IL-6, TNF-\u03b1, and especially IL-17. Additionally, analysis of the synovium from arthritic paws indicated that the same CD4+\/BV8+\/BV14+\/tetramer+ T cells were present in the arthritic joints. These data demonstrate that although only small numbers of CII-specific T cells are generated during the development of CIA, these cells express very high levels of cytokine mRNA and appear to preferentially migrate to the arthritic joint, indicating a potential direct role of CII-specific T cells in the pathogenesis of CIA.<\/jats:p>","DOI":"10.4049\/jimmunol.174.7.3978","type":"journal-article","created":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T02:39:15Z","timestamp":1398220755000},"page":"3978-3985","source":"Crossref","is-referenced-by-count":39,"title":["Ex Vivo Characterization of the Autoimmune T Cell Response in the HLA-DR1 Mouse Model of Collagen-Induced Arthritis Reveals Long-Term Activation of Type II Collagen-Specific Cells and Their Presence in Arthritic Joints"],"prefix":"10.1093","volume":"174","author":[{"given":"Kary A","family":"Latham","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Tennessee Health Science Center , Memphis, TN 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen B","family":"Whittington","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center , Memphis, TN 38104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruohong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Tennessee Health Science Center , Memphis, TN 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohui","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Tennessee Health Science Center , Memphis, TN 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward F","family":"Rosloniec","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center , Memphis, TN 38104"},{"name":"Department of Medicine, University of Tennessee Health Science Center , Memphis, TN 38163"},{"name":"Department of Pathology, University of Tennessee Health Science Center , Memphis, TN 38163"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,4,1]]},"reference":[{"key":"2025032706321078100_R1","first-page":"113","volume-title":"Immunol. 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