{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:52:26Z","timestamp":1789620746430,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The meningococcal class I outer membrane protein porin A plays an important role in the development of T cell-dependent protective immunity against meningococcal serogroup B infection and is therefore a major component of candidate meningococcal vaccines. T cell epitopes from porin A are poorly characterized because of weak in vitro memory T cell responses against purified Ag and strain variation. We applied a novel strategy to identify relevant naturally processed and MHC class II-presented porin A epitopes, based on stable isotope labeling of Ag. Human immature HLA-DR1-positive dendritic cells were used for optimal uptake and MHC class II processing of 14N- and 15N-labeled isoforms of the neisserial porin A serosubtype P1.5\u20132,10 in bacterial outer membrane vesicles. HLA-DR1 bound peptides, obtained after 48 h of Ag processing, contained typical spectral doublets in mass spectrometry that could easily be assigned to four porin A regions, expressed at diverging densities (\u223c30\u20134000 copies\/per cell). Epitopes from two of these regions are recognized by HLA-DR1-restricted CD4+ T cell lines and are conserved among different serosubtypes of meningococcal porin A. This mass tag-assisted approach provides a useful methodology for rapid identification of MHC class II presented bacterial CD4+ T cell epitopes relevant for vaccine development.<\/jats:p>","DOI":"10.4049\/jimmunol.174.9.5636","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T00:51:00Z","timestamp":1398127860000},"page":"5636-5643","source":"Crossref","is-referenced-by-count":20,"title":["Mass Tag-Assisted Identification of Naturally Processed HLA Class II-Presented Meningococcal Peptides Recognized by CD4+ T Lymphocytes"],"prefix":"10.1093","volume":"174","author":[{"given":"Hugo D","family":"Meiring","sequence":"first","affiliation":[{"name":"Laboratory of Analytical Chemistry, National Institute of Public Health and the Environment , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betsy","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline A M","family":"van Gaans-van den Brink","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martien C M","family":"Poelen","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Timmermans","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gino","family":"Baart","sequence":"additional","affiliation":[{"name":"Laboratorie of Product and Process Development, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humphrey","family":"Brugghe","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost","family":"van Schie","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire J P","family":"Boog","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ad P J M","family":"de Jong","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Chemistry, National Institute of Public Health and the Environment , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301cile A C M","family":"van Els","sequence":"additional","affiliation":[{"name":"Laboratorie of Vaccine Research, Netherlands Vaccine Institute , Bilthoven ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,5,1]]},"reference":[{"key":"2026020213022338700_R1","first-page":"1378","volume-title":"N. 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Immunol.","year":"2001"},{"key":"2026020213022338700_R12","first-page":"2891","volume-title":"Eur. J. Immunol.","year":"1991"},{"key":"2026020213022338700_R13","first-page":"871","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020213022338700_R14","first-page":"27","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020213022338700_R15","first-page":"178","volume-title":"Immunogenetics","year":"1995"},{"key":"2026020213022338700_R16","first-page":"3644","volume-title":"Eur. J. Immunol.","year":"2004"},{"key":"2026020213022338700_R17","first-page":"127","volume-title":"Mol. Immunol.","year":"2002"},{"key":"2026020213022338700_R18","first-page":"2926","volume-title":"Infect. Immun.","year":"2002"},{"key":"2026020213022338700_R19","first-page":"1449","volume-title":"J. Clin. Invest.","year":"1999"},{"key":"2026020213022338700_R20","first-page":"18549","volume-title":"J. Biol. Chem.","year":"1996"},{"key":"2026020213022338700_R21","first-page":"1391","volume-title":"J. Exp. Med.","year":"8003"},{"key":"2026020213022338700_R22","first-page":"1172","volume-title":"Eur. J. Immunol.","year":"2000"},{"key":"2026020213022338700_R23","first-page":"1602","volume-title":"Eur. J. Immunol.","year":"1993"},{"key":"2026020213022338700_R24","first-page":"1009","volume-title":"Vaccine","year":"1996"},{"key":"2026020213022338700_R25","first-page":"320","volume-title":"Acta Path. Microbiol. Scand.","year":"1959"},{"key":"2026020213022338700_R26","first-page":"1001","volume-title":"Vaccine","year":"1996"},{"key":"2026020213022338700_R27","first-page":"557","volume-title":"J. Sep. Sci.","year":"2002"},{"key":"2026020213022338700_R28","first-page":"1109","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026020213022338700_R29","first-page":"980","volume-title":"J. Virol.","year":"2003"},{"key":"2026020213022338700_R30","first-page":"429","volume-title":"J. 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