{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T09:20:49Z","timestamp":1791537649009,"version":"4.3.4"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hepatic expression levels of class I MHC Ags are generally regarded as very low. Because the status of these Ags and their ability to present peptides are important for the understanding of pathogen clearance and tolerogenic properties of the liver, we set out to identify the factors contributing to the reported phenotype. Unexpectedly, we found that the surface densities of Kb and Db on C57BL\/6 mouse hepatocytes are nearly as high as on splenocytes, as are the lysate concentrations of mRNA encoding H chain and \u03b22-microglobulin (\u03b22m). In contrast, the components of the peptide-loading pathway are reduced in hepatocytes. Despite the difference in the stoichiometric ratios of H chain\/\u03b22m\/peptide-loading machineries, both cell types express predominantly thermostable class I and are critically dependent on TAP and tapasin for display of surface Ags. Minor differences in the expression patterns in tapasin\u2212\/\u2212 background suggest cell specificity in class I assembly. Under immunostimulatory conditions, such as exposure to IFN-\u03b3 or Listeria monocytogenes, hepatocytes respond with a vigorous mRNA synthesis of the components of the Ag presentation pathway (up to 10-fold enhancement) but up-regulate H chain and \u03b22m to a lesser degree (&amp;lt;2-fold). This type of response should promote rapid influx of newly generated peptides into the endoplasmic reticulum and preferential presentation of foreign\/induced Ag by hepatic class I.<\/jats:p>","DOI":"10.4049\/jimmunol.175.2.1047","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T22:40:18Z","timestamp":1398206418000},"page":"1047-1055","source":"Crossref","is-referenced-by-count":45,"title":["Hepatocytes Express Abundant Surface Class I MHC and Efficiently Use Transporter Associated with Antigen Processing, Tapasin, and Low Molecular Weight Polypeptide Proteasome Subunit Components of Antigen Processing and Presentation Pathway"],"prefix":"10.1093","volume":"175","author":[{"given":"Ming","family":"Chen","sequence":"first","affiliation":[{"name":"Center for Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Tabaczewski","sequence":"additional","affiliation":[{"name":"Center for Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M","family":"Truscott","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine , St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Van Kaer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Vanderbilt University School of Medicine , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Stroynowski","sequence":"additional","affiliation":[{"name":"Center for Immunology, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center , Dallas, TX 75390"},{"name":"Department of Microbiology, University of Texas Southwestern Medical Center , Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,7,15]]},"reference":[{"key":"2026020212172380100_R1","first-page":"51","volume-title":"Nat. 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