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Recently, we showed that direct presentation of an epitope of the long-lived lymphocytic choriomeningitis virus nucleoprotein (LCMV-NP) required neosynthesis in accordance with the defective ribosomal products hypothesis. In this study, we report that LCMV-NP can be cross-primed in mice using either LCMV-NP-transfected human HEK293 or BALB\/c-derived B8 cells as Ag donor cells. In addition, we establish that contrary to direct presentation, cross-presentation required accumulation of the mature LCMV-NP and could not be sustained by the newly synthesized LCMV-NP protein, intermediate proteasomal degradation products, or the minimal NP396 epitope. Nevertheless, NP cross-presentation was enhanced by heat shock and was blunted by inhibitors of heat shock protein 90 and gp96. We propose that cross-presentation has evolved to sustain the presentation of stable viral proteins when their neosynthesis has ceased in infected donor cells.<\/jats:p>","DOI":"10.4049\/jimmunol.175.2.796","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T22:40:18Z","timestamp":1398206418000},"page":"796-805","source":"Crossref","is-referenced-by-count":61,"title":["Cross-Presentation of the Long-Lived Lymphocytic Choriomeningitis Virus Nucleoprotein Does Not Require Neosynthesis and Is Enhanced via Heat Shock Proteins"],"prefix":"10.1093","volume":"175","author":[{"given":"Sameh","family":"Basta","sequence":"first","affiliation":[{"name":"Department of Biology, Division of Immunology, University of Constance , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricarda","family":"Stoessel","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Immunology, University of Constance , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Basler","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Immunology, University of Constance , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maries","family":"van den Broek","sequence":"additional","affiliation":[{"name":"Institute of Experimental Immunology, Department of Pathology, University Hospital Zurich , Zurich ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Groettrup","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Immunology, University of Constance , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,7,15]]},"reference":[{"key":"2026020212170648700_R1","first-page":"1785","volume-title":"Proc. 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