{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:07:50Z","timestamp":1791248870159,"version":"4.1.0"},"reference-count":67,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>During thymic T cell development, immature CD4+CD8+ double-positive (DP) thymocytes develop either into CD4+CD8\u2212 Th cells or CD4\u2212CD8+ CTLs. Differentially expressed primary factors inducing the fate of these cell types are still poorly described. The transcription factor Runx3\/AML-2 Runx, rust dominant factor; AML, acute myeloid leukemia is expressed specifically during the development of CD8 single-positive (SP) thymocytes, where it silences CD4 expression. Deletion of murine Runx3 results in a reduction of CD8 SP T cells and concomitant accumulation of CD4+CD8+ T cells, which cannot down-regulate CD4 expression in the thymus and periphery. In this study we have investigated the role of Runx3 during thymocyte development and CD4 silencing and have identified integrin \u03b1E\/CD103 on CD8 SP T cells as a new potential target gene of Runx3. We demonstrate that Runx3 is necessary not only to repress CD4, but also to induce CD103 expression during development of CD8 SP T cells. In addition, transgenic overexpression of Runx3 reduced CD4 expression during development of DP thymocytes, leading to a reduced number of CD4 SP thymocytes and an increased number of CD8 SP thymocytes. This reversal is not caused by redirection of specific MHC class II-restricted cells to the CD8 lineage. Overexpression of Runx3 also up-regulated CD103 expression on a subpopulation of CD4 SP T cells with characteristics of regulatory T cells. Thus, Runx3 is a main regulator of CD4 silencing and CD103 induction and thus contributes to the phenotype of CD8 SP T cells during thymocyte development.<\/jats:p>","DOI":"10.4049\/jimmunol.175.3.1694","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T23:20:32Z","timestamp":1398036032000},"page":"1694-1705","source":"Crossref","is-referenced-by-count":111,"title":["Runx3 Regulates Integrin \u03b1E\/CD103 and CD4 Expression during Development of CD4\u2212\/CD8+ T Cells"],"prefix":"10.1093","volume":"175","author":[{"given":"Baerbel","family":"Grueter","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology (Cancer Research), University of Essen, Medical School , Essen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Petter","sequence":"additional","affiliation":[{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Egawa","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Skirball Institute of Biomolecular Medicine, New York University School of Medicine , New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Laule-Kilian","sequence":"additional","affiliation":[{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine J","family":"Aldrian","sequence":"additional","affiliation":[{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Wuerch","sequence":"additional","affiliation":[{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology (Cancer Research), University of Essen, Medical School , Essen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidehiro","family":"Fukuyama","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics and Immunology, The Rockefeller University , New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedda","family":"Wardemann","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, The Rockefeller University , New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Waldschuetz","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology (Cancer Research), University of Essen, Medical School , Essen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Mo\u0308ro\u0308y","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology (Cancer Research), University of Essen, Medical School , Essen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Taniuchi","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Skirball Institute of Biomolecular Medicine, New York University School of Medicine , New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Steimle","sequence":"additional","affiliation":[{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan R","family":"Littman","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Skirball Institute of Biomolecular Medicine, New York University School of Medicine , New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Ehlers","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology (Cancer Research), University of Essen, Medical School , Essen ,","place":["Germany"]},{"name":"Hans Spemann Laboratories, Max Planck Institute for Immunology , Freiburg ,","place":["Germany"]},{"name":"Laboratory of Molecular Genetics and Immunology, The Rockefeller University , New York, NY 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,8,1]]},"reference":[{"key":"2026020214052537700_R1","first-page":"139","volume-title":"Annu. 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