{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:23:07Z","timestamp":1790648587270,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cytotoxic lymphocytes express a large family of granule serine proteases, including one member, granzyme (Grz)M, with a unique protease activity, restricted expression, and distinct gene locus. Although a number of Grzs, including GrzM, have been shown to mediate target cell apoptosis in the presence of perforin, the biological activity of Grz has been restricted to control of a number of viral pathogens, including two natural mouse pathogens, ectromelia, and murine CMV (MCMV). In this article, we describe the first reported gene targeting of GrzM in mice. GrzM-deficient mice display normal NK cell\/T cell development and homeostasis and intact NK cell-mediated cytotoxicity of tumor targets as measured by membrane damage and DNA fragmentation. GrzM-deficient mice demonstrated increased susceptibility to MCMV infection typified by the presence of more viral inclusions and transiently higher viral burden in the visceral organs of GrzM-deficient mice compared with wild-type (WT) mice. The cytotoxicity of NK cells from MCMV-infected GrzM-deficient mice remained unchanged and, like WT control mice, GrzM-deficient mice eventually effectively cleared MCMV infection from the visceral organs. In contrast, GrzM-deficient mice were as resistant as WT control mice to mouse pox ectromelia infection, as well as challenge with a number of NK cell-sensitive tumors. These data confirm a role for GrzM in the host response to MCMV infection, but suggest that GrzM is not critical for NK cell-mediated cytotoxicity.<\/jats:p>","DOI":"10.4049\/jimmunol.175.5.3235","type":"journal-article","created":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T13:44:04Z","timestamp":1398347044000},"page":"3235-3243","source":"Crossref","is-referenced-by-count":61,"title":["Functional Analysis of Granzyme M and Its Role in Immunity to Infection"],"prefix":"10.1093","volume":"175","author":[{"given":"Lily I","family":"Pao","sequence":"first","affiliation":[{"name":"Cancer Biology Program, Division of Hematology-Oncology, Beth Israel-Deaconess Medical Center and Havard Medical School , Boston, MA 02215"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nital","family":"Sumaria","sequence":"additional","affiliation":[{"name":"Immunology and Virology Program, Centre for Opthalmology and Visual Science, The University of Western Australia , Crawley, Western Australia ,","place":["Australia"]},{"name":"Centre for Experimental Immunology, Lions Eye Institute , Nedlands, Western Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice M","family":"Kelly","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre , East Melbourne, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serani","family":"van Dommelen","sequence":"additional","affiliation":[{"name":"Immunology and Virology Program, Centre for Opthalmology and Visual Science, The University of Western Australia , Crawley, Western Australia ,","place":["Australia"]},{"name":"Centre for Experimental Immunology, Lions Eye Institute , Nedlands, Western Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Cretney","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre , East Melbourne, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan E","family":"Wallace","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre , East Melbourne, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree A","family":"Anthony","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre , East Melbourne, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P","family":"Uldrich","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne , Parkville, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale I","family":"Godfrey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne , Parkville, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M","family":"Papadimitriou","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Western Australia , Crawley, Western Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arno","family":"Mullbacher","sequence":"additional","affiliation":[{"name":"Division of Immunology and Genetics, John Curtain School of Medical Research, Australian National University , Canberra ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariapia A","family":"Degli-Esposti","sequence":"additional","affiliation":[{"name":"Immunology and Virology Program, Centre for Opthalmology and Visual Science, The University of Western Australia , Crawley, Western Australia ,","place":["Australia"]},{"name":"Centre for Experimental Immunology, Lions Eye Institute , Nedlands, Western Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J","family":"Smyth","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Trescowthick Laboratories, Peter MacCallum Cancer Centre , East Melbourne, Victoria ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,9,1]]},"reference":[{"key":"2025032705425969600_R1","first-page":"850","volume-title":"Nat. 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