{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T04:22:54Z","timestamp":1775190174325,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The granule-derived mouse mast cell proteases-1 and -2 (mMCP-1 and -2) colocalize in similar quantities in mucosal mast cells but micrograms of mMCP-1 compared with nanograms of mMCP-2 are detected in peripheral blood during intestinal nematode infection. This differential systemic response was investigated both in vitro and in vivo. Bone marrow-derived mucosal mast cell homologs released similar quantities of mMCP-1 and-2 concomitantly with \u03b2-hexosaminidase in response to calcium ionophore (\u223c60% release) or IgE\/DNP (25% release). In contrast, serum from mice sensitized by infection with Nippostrongylus brasiliensis 10 days earlier contained &amp;gt;1500-fold more mMCP-1 (10,130 \u00b1 1,609 ng\/ml) than mMCP-2 (6.4 \u00b1 1 ng\/ml), but, in gut lumen, the difference was \u223c8-fold. After OVA sensitization, &amp;gt;600-fold more mMCP-1 (7,861 \u00b1 2,209 ng\/ml) than mMCP-2 (12.8 \u00b1 4.7 ng\/ml) was present in blood 1 h after challenge, but, in gut lumen, there were relatively comparable levels of mMCP-1 and -2. To estimate the rates of systemic accumulation and clearance, 10 \u03bcg of mMCP-1 or -2 was injected i.p. Plasma levels of injected mMCP-2 peaked (1%) at 15 min then declined, whereas levels of mMCP-1 were maximal (\u223c25%) at 3 h. Inactivation of mMCP-1 with PMSF before injection resulted in mMCP-2-like kinetics, but inhibition of mMCP-1 by serum gave kinetics similar to that of native mMCP-1. mMCP-1 isolated from serum is complexed with serpins and we conclude that both the accumulation and the longevity of mMCP-1 in blood is due to complex formation, protecting it from a pathway that rapidly clears mMCP-2, which is unable to form complexes with serpins.<\/jats:p>","DOI":"10.4049\/jimmunol.176.2.899","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:43:16Z","timestamp":1398022996000},"page":"899-904","source":"Crossref","is-referenced-by-count":22,"title":["Anaphylactic Release of Mucosal Mast Cell Granule Proteases: Role of Serpins in the Differential Clearance of Mouse Mast Cell Proteases-1 and -2"],"prefix":"10.1093","volume":"176","author":[{"given":"Alan D","family":"Pemberton","sequence":"first","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven H","family":"Wright","sequence":"additional","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela A","family":"Knight","sequence":"additional","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugh R P","family":"Miller","sequence":"additional","affiliation":[{"name":"Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush Veterinary Centre , Roslin, Midlothian ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,1,15]]},"reference":[{"key":"2026020211310941200_R1","first-page":"1","volume-title":"Monogr. 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