{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:56:50Z","timestamp":1781333810080,"version":"3.54.1"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Deciphering how Listeria monocytogenes exploits the host cell machinery to invade mammalian cells is a key issue in understanding the pathogenesis of this food-borne pathogen, which can cause diseases ranging from gastroenteritis to meningitis and abortion. In this study, we show that the lysosomal aspartyl-protease cathepsin-D (Ctsd) is of considerable importance for nonoxidative listericidal defense mechanisms. We observed enhanced susceptibility to L. monocytogenes infection of fibroblasts and bone-marrow macrophages and increased intraphagosomal viability of bacteria in fibroblasts isolated from Ctsd-deficient mice compared with wild type. These findings are further supported by prolonged survival of L. monocytogenes in Ctsd-deficient mice after infection. Transient transfection of Ctsd in wild-type cells was sufficient to revert these wild-type phagosomes back to microbicidal compartments. Based on infection experiments with mutant bacteria, in vitro degradation, and immunoprecipitation experiments, we suggest that a major target of cathepsin D is the main virulence factor listeriolysin O.<\/jats:p>","DOI":"10.4049\/jimmunol.176.3.1321","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T18:20:05Z","timestamp":1398018005000},"page":"1321-1325","source":"Crossref","is-referenced-by-count":46,"title":["Cutting Edge: A Novel Nonoxidative Phagosomal Mechanism Exerted by Cathepsin-D Controls\n                    <i>Listeria monocytogenes<\/i>\n                    Intracellular Growth"],"prefix":"10.1093","volume":"176","author":[{"given":"Elida","family":"del Cerro-Vadillo","sequence":"first","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fidel","family":"Madrazo-Toca","sequence":"additional","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenio","family":"Carrasco-Mari\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Fernandez-Prieto","sequence":"additional","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Beck","sequence":"additional","affiliation":[{"name":"Biochemisches Institut, Christian-Albrechts-Universita\u0308t Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Leyva-Cobia\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Saftig","sequence":"additional","affiliation":[{"name":"Biochemisches Institut, Christian-Albrechts-Universita\u0308t Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Alvarez-Dominguez","sequence":"additional","affiliation":[{"name":"Servicio de Inmunologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Servicio Ca\u0301ntabro de Salud , Santander ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,2,1]]},"reference":[{"key":"2026020214081067800_R1","first-page":"409","volume-title":"J. 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