{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:17:16Z","timestamp":1790259436911,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Transcriptional silencing mediated by DNA methylation is a critical component of epigenetic regulation during early embryonic development in animals. However, the requirement for DNA methylation during activation and differentiation of mature CD8+ T cells into effector and memory cells is not clear. Using cre-mediated deletion of DNA methyltransferase 1 (Dnmt1) at the time of CD8+ T cell activation, we investigated the obligation for maintaining patterns of DNA methylation during the generation of Ag-specific effector and memory CD8+ T cells in response to acute viral infection of mice with lymphocytic choriomeningitis virus. Dnmt1\u2212\/\u2212 CD8+ T cells failed to undergo the massive CD8+ T cell expansion characteristic of lymphocytic choriomeningitis virus infection, leading to &amp;gt;80% reductions in Ag-specific effector CD8+ T cells at the height of the response. Despite this, Dnmt1\u2212\/\u2212 CD8+ T cells efficiently controlled the viral infection. Interestingly, the number of Ag-specific Dnmt1\u2212\/\u2212 memory CD8+ T cells was moderately reduced compared with the reductions seen at day 8 postinfection. Our data suggest that ablation of Dnmt1 and subsequent DNA methylation affect the finite proliferative potential of Ag-specific CD8+ T cells with moderate effects on their differentiation to effector and memory CD8+ T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.176.8.4562","type":"journal-article","created":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T20:59:46Z","timestamp":1398286786000},"page":"4562-4572","source":"Crossref","is-referenced-by-count":48,"title":["DNA Methylation by DNA Methyltransferase 1 Is Critical for Effector CD8 T Cell Expansion"],"prefix":"10.1093","volume":"176","author":[{"given":"Craig","family":"Chappell","sequence":"first","affiliation":[{"name":"Emory Vaccine Center, Department of Microbiology and Immunology, Emory University , Atlanta, GA 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Beard","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology , Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Altman","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center, Department of Microbiology and Immunology, Emory University , Atlanta, GA 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolph","family":"Jaenisch","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology , Cambridge, MA 02139"},{"name":"Biology Department, Massachusetts Institute of Technology , Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshy","family":"Jacob","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center, Department of Microbiology and Immunology, Emory University , Atlanta, GA 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,4,15]]},"reference":[{"key":"2026020211320222700_R1","first-page":"333","volume-title":"Annu. 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