{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:49:46Z","timestamp":1790646586899,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Somatic hypermutation (SHM) of the rearranged Ig genes is required for the affinity maturation of Abs. SHM is almost exclusively targeted to the rearranged Ig loci, but the mechanism of this gene-specific targeting remains unclear. The Ig \u03ba L chain locus contains multiple enhancers, including the MAR\/intronic (iE\u03ba) and 3\u2032 enhancers (3\u2032E\u03ba). Previous transgenic studies indicate that both \u03ba enhancers are individually necessary for SHM of Igk. In contrast, later studies of Ag-selected V\u03ba genes in 3\u2032E\u03ba\u2212\/\u2212 mice found no absolute requirement for 3\u2032E\u03ba in \u03ba SHM. To address the roles of the two \u03ba enhancers in SHM in a physiological context, we analyzed SHM of the endogenous Igk in mice with a targeted deletion of either iE\u03ba or 3\u2032E\u03ba in Peyer\u2019s patch germinal center B cells. Our findings indicate that, although 3\u2032E\u03ba is quantitatively important for SHM of Igk, iE\u03ba is not required for \u03ba SHM. In addition, a reduction of \u03ba mRNA levels is also detected in activated 3\u2032E\u03ba\u2212\/\u2212 B cells. These findings suggest that iE\u03ba and 3\u2032E\u03ba play distinct roles in regulating Igk transcription and SHM.<\/jats:p>","DOI":"10.4049\/jimmunol.177.2.1146","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T23:19:01Z","timestamp":1397863141000},"page":"1146-1151","source":"Crossref","is-referenced-by-count":41,"title":["Roles of the Ig \u03ba Light Chain Intronic and 3\u2032 Enhancers in <i>Igk<\/i> Somatic Hypermutation"],"prefix":"10.1093","volume":"177","author":[{"given":"Matthew A","family":"Inlay","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather H","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie H","family":"Odegard","sequence":"additional","affiliation":[{"name":"Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine , New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongxiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G","family":"Schatz","sequence":"additional","affiliation":[{"name":"Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine , New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of California , San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,7,15]]},"reference":[{"key":"2025032613401311800_R1","first-page":"S45","volume-title":"Cell","year":"2002"},{"key":"2025032613401311800_R2","first-page":"165","volume-title":"Annu. 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