{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:23:12Z","timestamp":1790407392162,"version":"4.1.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Live attenuated Shigella vaccines elicit protective immune responses, but involve a potential risk of inducing a strong inflammatory reaction. The bacterial invasiveness that is crucial for Ag delivery causes inflammatory destruction of infected epithelial cells and proinflammatory cell death of infected macrophages. In this study, the noninvasive Shigella mutant \u0394ipaB was equipped with Yersinia invasin protein, which has been shown to mediate bacterial invasion and targeting to M cells located in follicle-associated epithelium. Invasin-expressing \u0394ipaB (\u0394ipaB\/inv) was internalized into epithelial cells and retained in the intraphagosomal space. \u0394ipaB\/inv did not induce necrotic cell death of infected macrophages nor cause symptomatic damage after intranasal vaccination of mice. \u0394ipaB\/inv was safer and more effective than the conventional live vaccine, \u0394virG. Infection by \u0394ipaB\/inv caused polymorphonuclear neutrophil infiltration in the lung, but did not induce production of large amounts of proinflammatory cytokines. We concluded that the low experimental morbidity and high vaccine efficacy of \u0394ipaB\/inv are primarily based on high protective immune responses, which may be enhanced by the polymorphonuclear neutrophil infiltration unaccompanied by tissue injury.<\/jats:p>","DOI":"10.4049\/jimmunol.177.7.4709","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T13:15:01Z","timestamp":1397826901000},"page":"4709-4717","source":"Crossref","is-referenced-by-count":27,"title":["High Vaccine Efficacy against Shigellosis of Recombinant Noninvasive\n                    <i>Shigella<\/i>\n                    Mutant That Expresses\n                    <i>Yersinia<\/i>\n                    Invasin"],"prefix":"10.1093","volume":"177","author":[{"given":"Toshihiko","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]},{"name":"Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency , Kawaguchi ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ashida","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Iwai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahito","family":"Toyotome","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenori","family":"Matsui","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Immunology, Kitasato Institute for Life Sciences, Kitasato University , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Sasakawa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]},{"name":"Department of Infectious Disease Control, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo , Tokyo ,","place":["Japan"]},{"name":"Core Research for Evolutional Science and Technology, Japan Science and Technology Agency , Kawaguchi ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,10,1]]},"reference":[{"key":"2026020209083288600_R1","first-page":"651","volume-title":"Bull. 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