{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T03:11:32Z","timestamp":1770088292151,"version":"3.49.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>T cells use the vascular adhesion molecules E- and P-selectin to enter inflamed skin. Previous studies have indicated the possibility for diversity in the synthesis of E- and P-selectin glycan ligands by activated T cells due to their different requirements for the O-glycan branching enzyme core 2 \u03b21,6-N-acetylglucosaminyltransferase I and its independent regulation. It is known that T cell staining by the mAb HECA-452 (referred to as cutaneous lymphocyte-associated Ag (CLA) T cells) correlates with E-selectin binding, yet whether these cells uniformly bind P-selectin is less clear. The mAb CHO-131 and P-selectin binding require a glycan moiety consisting of a sialylated and fucosylated oligosaccharide properly positioned on a core-2 O-glycan. Interestingly, CHO-131 stains a subset of CLA+ T cells. A direct comparison of the selectin binding capacity of CHO-131+ and CHO-131\u2212 CLA+ T cells revealed a significantly greater P-selectin, but not E-selectin, binding activity by the former subset. Based on the expression of homing and central and effector memory cell markers, CHO-131+ and CHO-131\u2212 CLA+ T cells have an overlapping skin-tropic and memory phenotype. CHO-131+ T cells were considerably enriched in psoriatic skin, yet, unlike the peripheral blood of healthy individuals, HECA-452 and CHO-131 stained a similar proportion of T cells in the cutaneous lesions, indicating an accumulation advantage by CHO-131+ T cells. We conclude that the CHO-131+CLA+ T cell subset is enriched in P-selectin binding cells. These findings should provide new insights into the regulation and function of skin homing T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.177.7.4742","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T13:15:01Z","timestamp":1397826901000},"page":"4742-4748","source":"Crossref","is-referenced-by-count":7,"title":["The Monoclonal Antibody CHO-131 Identifies a Subset of Cutaneous Lymphocyte-Associated Antigen T Cells Enriched in P-Selectin-Binding Cells"],"prefix":"10.1093","volume":"177","author":[{"given":"Zhenya","family":"Ni","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota , St. Paul, MN 55108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, Children\u2019s Hospital Boston , Boston, MA 02115"},{"name":"Joint Program in Transfusion Medicine, Children\u2019s Hospital Boston , Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Niehans","sequence":"additional","affiliation":[{"name":"Minneapolis Department of Veterans Affairs Medical Center , Minneapolis, MN 55417"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce","family":"Walcheck","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Minnesota , St. Paul, MN 55108"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,10,1]]},"reference":[{"key":"2026020208195302100_R1","first-page":"1","volume-title":"Immunity","year":"2002"},{"key":"2026020208195302100_R2","first-page":"978","volume-title":"Nature","year":"1997"},{"key":"2026020208195302100_R3","first-page":"325","volume-title":"Nat. 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Immunol.","year":"2003"},{"key":"2026020208195302100_R13","first-page":"1677","volume-title":"Blood","year":"2003"},{"key":"2026020208195302100_R14","first-page":"1556","volume-title":"Am. J. Pathol.","year":"1993"},{"key":"2026020208195302100_R15","first-page":"394","volume-title":"Curr. Opin. Immunol.","year":"1994"},{"key":"2026020208195302100_R16","first-page":"537","volume-title":"J. Clin. Invest.","year":"2002"},{"key":"2026020208195302100_R17","first-page":"1461","volume-title":"J. Exp. Med.","year":"1991"},{"key":"2026020208195302100_R18","first-page":"14869","volume-title":"J. Biol. Chem.","year":"1991"},{"key":"2026020208195302100_R19","first-page":"911","volume-title":"J. Cell Biol.","year":"1996"},{"key":"2026020208195302100_R20","first-page":"3806","volume-title":"Blood","year":"2001"},{"key":"2026020208195302100_R21","first-page":"4476","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020208195302100_R22","first-page":"628","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020208195302100_R23","first-page":"4063","volume-title":"Blood","year":"2002"},{"key":"2026020208195302100_R24","first-page":"18732","volume-title":"J. Biol. Chem.","year":"1996"},{"key":"2026020208195302100_R25","first-page":"24838","volume-title":"J. Biol. Chem.","year":"1999"},{"key":"2026020208195302100_R26","first-page":"37987","volume-title":"J. Biol. Chem.","year":"2003"},{"key":"2026020208195302100_R27","first-page":"21984","volume-title":"J. Biol. Chem.","year":"2004"},{"key":"2026020208195302100_R28","first-page":"717","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020208195302100_R29","first-page":"464","volume-title":"J. Leukocyte Biol.","year":"2003"},{"key":"2026020208195302100_R30","first-page":"720","volume-title":"Nature","year":"1996"},{"key":"2026020208195302100_R31","first-page":"105","volume-title":"Cancer Lett.","year":"2005"},{"key":"2026020208195302100_R32","first-page":"C323","volume-title":"Am. J. Physiol.","year":"2005"},{"key":"2026020208195302100_R33","first-page":"796","volume-title":"Nature","year":"1991"},{"key":"2026020208195302100_R34","first-page":"21966","volume-title":"J. Biol. Chem.","year":"1995"},{"key":"2026020208195302100_R35","first-page":"1669","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020208195302100_R36","first-page":"280","volume-title":"Exp. Dermatol.","year":"2001"},{"key":"2026020208195302100_R37","first-page":"246","volume-title":"Arch. Dermatol. Res.","year":"1998"},{"key":"2026020208195302100_R38","first-page":"2426","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020208195302100_R39","first-page":"708","volume-title":"Nature","year":"1999"},{"key":"2026020208195302100_R40","first-page":"245","volume-title":"Nat. Med.","year":"2001"},{"key":"2026020208195302100_R41","first-page":"877","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020208195302100_R42","first-page":"717","volume-title":"J. 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