{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:48:04Z","timestamp":1790873284838,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent studies on Hodgkin\u2019s lymphoma (HL) have indicated that patients with active disease display functional impairment of Ag-specific CD8+ T cells due to expansion of regulatory T cells at sites of disease and in the peripheral blood. Adoptive cellular immunotherapy based on EBV-specific CD8+ T cells has been explored with limited success to date. It has been proposed that improved targeting of these CD8+ T cells toward viral Ags that are expressed in HL may enhance future therapeutic vaccine strategies. In this study, we have developed a novel replication-deficient adenoviral Ag presentation system that is designed to encode glycine alanine repeat-deleted EBV nuclear Ag 1 covalently linked to multiple CD8+ T cell epitopes from latent membrane proteins 1 and 2. A single stimulation of CD8+ T cells from healthy virus carriers, and patients with HL with this adenoviral construct in combination with IL-2, was sufficient to reverse the functional T cell impairment and restored both IFN-\u03b3 production and cytolytic function. More importantly, these activated CD8+ T cells responded to tumor cells expressing membrane proteins and recognized novel EBNA1 epitopes. Flow cytometric analysis revealed that a large proportion of T cells expanded from patients with HL were CD62Lhigh and CD27high, and CCR7low, consistent with early to mid effector T cells. These findings provide an important platform for translation of Ag-specific adoptive immunotherapy for the treatment of EBV-associated malignancies such as HL and nasopharyngeal carcinoma.<\/jats:p>","DOI":"10.4049\/jimmunol.177.7.4897","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T13:15:01Z","timestamp":1397826901000},"page":"4897-4906","source":"Crossref","is-referenced-by-count":61,"title":["Functional Reversion of Antigen-Specific CD8+ T Cells from Patients with Hodgkin Lymphoma following In Vitro Stimulation with Recombinant Polyepitope"],"prefix":"10.1093","volume":"177","author":[{"given":"Corey","family":"Smith","sequence":"first","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne","family":"Cooper","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"Burgess","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Rist","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Webb","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanore","family":"Lambley","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Tellam","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Marlton","sequence":"additional","affiliation":[{"name":"Department of Haematology, Princess Alexandra Hospital , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F","family":"Seymour","sequence":"additional","affiliation":[{"name":"Haematology Service, Peter MacCallum Cancer Centre, and University of Melbourne , Melbourne ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]},{"name":"Department of Haematology, Princess Alexandra Hospital , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv","family":"Khanna","sequence":"additional","affiliation":[{"name":"Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research , Brisbane ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,10,1]]},"reference":[{"key":"2026020209024555900_R1","first-page":"1","volume-title":"Leuk. 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