{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:20:04Z","timestamp":1783624804214,"version":"3.55.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Using noncompetitive methodologies comparing CD43+\/+ and CD43\u2212\/\u2212 mice, it has been reported that CD43\u2212\/\u2212 leukocytes exhibit reduced recruitment efficiency to sites of inflammation. More recent analyses demonstrate that CD43 on activated T cells can function as an E-selectin ligand (E-SelL) in vitro, suggesting that CD43 might promote rolling interactions during recruitment of leukocytes and account for the reported recruitment deficits in CD43\u2212\/\u2212 T cells and neutrophils in vivo. Internally controlled competitive in vivo methods using fluorescent tracking dyes were applied to compare recruitment efficiency of CD43+\/+ vs CD43\u2212\/\u2212 activated T cells to inflamed skin and of peripheral blood neutrophils to inflamed peritoneum. A simple CFSE perfusion method was developed to distinguish arterial\/venous vasculature and confirm appropriate extravasation through venules in a Con A-induced cutaneous inflammation model. In vivo recruitment of peripheral blood neutrophils to inflamed peritoneum was core 2 GlcNAcT-I dependent, but recruitment efficiency was not influenced by absence of CD43. There were also no significant differences in core 2 GlcNAcT-I-dependent, selectin-dependent, cutaneous recruitment of activated T cells from CD43+\/+ and congenic CD43\u2212\/\u2212 mice in either B6 or P-selectin\u2212\/\u2212 recipients despite biochemical confirmation that a CD43-specific E-SelL was present on activated T cells. We conclude that recruitment of neutrophils and activated T cells in these in vivo models is not influenced by CD43 expression and that if CD43 on activated T cells performs an E-SelL function in vivo, it contributes in a limited physiological context.<\/jats:p>","DOI":"10.4049\/jimmunol.177.9.6450","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:02:17Z","timestamp":1397847737000},"page":"6450-6459","source":"Crossref","is-referenced-by-count":17,"title":["CD43 Deficiency Has No Impact in Competitive In Vivo Assays of Neutrophil or Activated T Cell Recruitment Efficiency"],"prefix":"10.1093","volume":"177","author":[{"given":"Douglas A","family":"Carlow","sequence":"first","affiliation":[{"name":"Biomedical Research Centre and Department of Pathology and Laboratory Medicine, University of British Columbia , British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann J","family":"Ziltener","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre and Department of Pathology and Laboratory Medicine, University of British Columbia , British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,11,1]]},"reference":[{"key":"2026020211135454900_R1","first-page":"325","volume-title":"Nat. Rev. Immunol.","year":"2004"},{"key":"2026020211135454900_R2","first-page":"444","volume-title":"Curr. Opin. Hematol.","year":"2005"},{"key":"2026020211135454900_R3","first-page":"3259","volume-title":"Blood","year":"1996"},{"key":"2026020211135454900_R4","first-page":"263","volume-title":"Trends Mol. Med.","year":"2003"},{"key":"2026020211135454900_R5","first-page":"665","volume-title":"Immunity","year":"2000"},{"key":"2026020211135454900_R6","first-page":"881","volume-title":"Immunity","year":"1998"},{"key":"2026020211135454900_R7","first-page":"19","volume-title":"Immunol. Rev.","year":"2002"},{"key":"2026020211135454900_R8","first-page":"1","volume-title":"Leuk. Lymphoma","year":"1998"},{"key":"2026020211135454900_R9","first-page":"1765","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020211135454900_R10","first-page":"963","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020211135454900_R11","first-page":"5645","volume-title":"J. Immunol.","year":"2002"},{"key":"2026020211135454900_R12","first-page":"1669","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020211135454900_R13","first-page":"8042","volume-title":"J. Immunol.","year":"2005"},{"key":"2026020211135454900_R14","first-page":"1421","volume-title":"Blood","year":"2006"},{"key":"2026020211135454900_R15","first-page":"2181","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020211135454900_R16","first-page":"1493","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020211135454900_R17","first-page":"5678","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020211135454900_R18","first-page":"6022","volume-title":"J. Immunol.","year":"2002"},{"key":"2026020211135454900_R19","first-page":"6527","volume-title":"J. Immunol.","year":"2003"},{"key":"2026020211135454900_R20","first-page":"535","volume-title":"Nature","year":"1995"},{"key":"2026020211135454900_R21","first-page":"256","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020211135454900_R22","first-page":"2329","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020211135454900_R23","first-page":"3959","volume-title":"J. Immunol.","year":"2005"},{"key":"2026020211135454900_R24","first-page":"968","volume-title":"Indian J. Exp. Biol.","year":"1996"},{"key":"2026020211135454900_R25","first-page":"3426","volume-title":"J. Immunol.","year":"1994"},{"key":"2026020211135454900_R26","first-page":"3111","volume-title":"Blood","year":"2004"},{"key":"2026020211135454900_R27","first-page":"643","volume-title":"J. Exp. Med.","year":"2003"},{"key":"2026020211135454900_R28","first-page":"1934","volume-title":"J. Immunol.","year":"2002"},{"key":"2026020211135454900_R29","first-page":"751","volume-title":"Int. Immunol.","year":"2002"},{"key":"2026020211135454900_R30","first-page":"3806","volume-title":"Blood","year":"2001"},{"key":"2026020211135454900_R31","first-page":"3812","volume-title":"Blood","year":"2001"},{"key":"2026020211135454900_R32","first-page":"1034","volume-title":"Circ. Res.","year":"1991"},{"key":"2026020211135454900_R33","first-page":"127","volume-title":"Microvasc. Res.","year":"1992"},{"key":"2026020211135454900_R34","first-page":"92","volume-title":"J. Clin. Invest.","year":"1989"},{"key":"2026020211135454900_R35","first-page":"152","volume-title":"Thromb. Haemostasis","year":"1993"},{"key":"2026020211135454900_R36","first-page":"463","volume-title":"Microcirculation","year":"2003"},{"key":"2026020211135454900_R37","first-page":"139","volume-title":"J. Immunol. Methods","year":"1996"},{"key":"2026020211135454900_R38","first-page":"24379","volume-title":"J. Biol. Chem.","year":"1992"},{"key":"2026020211135454900_R39","first-page":"6841","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020211135454900_R40","first-page":"2169","volume-title":"J. Immunol.","year":"1987"},{"key":"2026020211135454900_R41","first-page":"11054","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1993"},{"key":"2026020211135454900_R42","first-page":"534","volume-title":"Immunology","year":"1997"},{"key":"2026020211135454900_R43","first-page":"213","volume-title":"Br. J. Plast. Surg.","year":"1984"},{"key":"2026020211135454900_R44","first-page":"643","volume-title":"Cell","year":"1996"},{"key":"2026020211135454900_R45","first-page":"581","volume-title":"Annu. Rev. Cell Dev. Biol.","year":"2005"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/177\/9\/6450\/62651153\/zim02106006450.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/177\/9\/6450\/62651153\/zim02106006450.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T16:14:01Z","timestamp":1770048841000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/177\/9\/6450\/8031692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,1]]},"references-count":45,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2006,11,1]]},"published-print":{"date-parts":[[2006,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.177.9.6450","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2006,11]]},"published":{"date-parts":[[2006,11,1]]}}}