{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:11:24Z","timestamp":1791155484673,"version":"4.1.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mouse models of allergen provocation and\/or transgenic gene expression have provided significant insights regarding the cellular, molecular, and immune responses linked to the pathologies occurring as a result of allergic respiratory inflammation. Nonetheless, the inability to replicate the eosinophil activities occurring in patients with asthma has limited their usefulness to understand the larger role(s) of eosinophils in disease pathologies. These limitations have led us to develop an allergen-naive double transgenic mouse model that expresses IL-5 systemically from mature T cells and eotaxin-2 locally from lung epithelial cells. We show that these mice develop several pulmonary pathologies representative of severe asthma, including structural remodeling events such as epithelial desquamation and mucus hypersecretion leading to airway obstruction, subepithelial fibrosis, airway smooth muscle hyperplasia, and pathophysiological changes exemplified by exacerbated methacholine-induced airway hyperresponsiveness. More importantly, and similar to human patients, the pulmonary pathologies observed are accompanied by extensive eosinophil degranulation. Genetic ablation of all eosinophils from this double transgenic model abolished the induced pulmonary pathologies, demonstrating that these pathologies are a consequence of one or more eosinophil effector functions.<\/jats:p>","DOI":"10.4049\/jimmunol.178.12.7879","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T23:18:11Z","timestamp":1397863091000},"page":"7879-7889","source":"Crossref","is-referenced-by-count":137,"title":["Coexpression of IL-5 and Eotaxin-2 in Mice Creates an Eosinophil-Dependent Model of Respiratory Inflammation with Characteristics of Severe Asthma"],"prefix":"10.1093","volume":"178","author":[{"given":"Sergei I","family":"Ochkur","sequence":"first","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl A","family":"Protheroe","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis L","family":"Biechele","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph S","family":"Pero","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P","family":"McGarry","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Second Hospital, Zhejiang University College of Medicine , HangZhou ,","place":["People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie R","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana C","family":"Colbert","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas V","family":"Colby","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine\/Pathology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huahao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Second Hospital, Zhejiang University College of Medicine , HangZhou ,","place":["People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Texas Houston Health Science Center , Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles C","family":"Irvin","sequence":"additional","affiliation":[{"name":"Vermont Lung Center, Department of Medicine, University of Vermont , Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy A","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona , Scottsdale, AZ 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,6,15]]},"reference":[{"key":"2025032803195279000_R1","first-page":"11","volume-title":"Lancet","year":"1982"},{"key":"2025032803195279000_R2","first-page":"286","volume-title":"J. 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