{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:14:25Z","timestamp":1790489665584,"version":"4.1.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Similar to Ipaf and caspase-1, the Nod-like receptor protein Naip5 restricts intracellular proliferation of Legionella pneumophila, the causative agent of a severe form of pneumonia known as Legionnaires\u2019 disease. Thus, Naip5 has been suggested to regulate Legionella replication inside macrophages through the activation of caspase-1. In this study, we show that cytosolic delivery of recombinant flagellin activated caspase-1 in A\/J macrophages carrying a mutant Naip5 allele, and in C57BL\/6 (B6) macrophages congenic for the mutant Naip5 allele (B6-Naip5A\/J), but not in Ipaf\u2212\/\u2212 cells. In line with these results, A\/J and B6-Naip5A\/J macrophages induced high levels of caspase-1 activation and IL-1\u03b2 secretion when infected with Legionella. In addition, transgenic expression of a functional Naip5 allele in A\/J macrophages did not alter Legionella-induced caspase-1 activation and IL-1\u03b2 secretion. Notably, defective Naip5 signaling renders B6-Naip5A\/J macrophages permissive for Legionella proliferation despite normal caspase-1 activation. These results indicate that the restriction of intracellular Legionella replication is more complex than previously appreciated and requires both Ipaf-dependent caspase-1 activation as well as functional Naip5 signaling.<\/jats:p>","DOI":"10.4049\/jimmunol.178.12.8022","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:18:11Z","timestamp":1397848691000},"page":"8022-8027","source":"Crossref","is-referenced-by-count":88,"title":["The Nod-Like Receptor Family Member Naip5\/Birc1e Restricts\n                    <i>Legionella pneumophila<\/i>\n                    Growth Independently of Caspase-1 Activation"],"prefix":"10.1093","volume":"178","author":[{"given":"Mohamed","family":"Lamkanfi","sequence":"first","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Amer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thirumala-Devi","family":"Kanneganti","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rau\u0301l","family":"Mun\u0303oz-Planillo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vandenabeele","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Research, Molecular Signalling and Cell Death Unit, Flanders Institute for Biotechnology and Ghent University , Zwijnaarde ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Fortier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Center for the Study of Host Resistance, McGill University , Montreal, Quebec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Gros","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Center for the Study of Host Resistance, McGill University , Montreal, Quebec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Nu\u0301n\u0303ez","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,6,15]]},"reference":[{"key":"2025032803200670400_R1","first-page":"506","volume-title":"Clin. 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