{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:33:43Z","timestamp":1792373623968,"version":"4.4.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The gut provides a large area for immunization enabling the development of mucosal and systemic Ab responses. To test whether the protective Ags to Yersinia pestis can be orally delivered, the Y. pestis caf1 operon, encoding the F1-Ag and virulence Ag (V-Ag) were cloned into attenuated Salmonella vaccine vectors. F1-Ag expression was controlled under a promoter from the caf1 operon; two different promoters (P), PtetA in pV3, PphoP in pV4, as well as a chimera of the two in pV55 were tested. F1-Ag was amply expressed; the chimera in the pV55 showed the best V-Ag expression. Oral immunization with Salmonella-F1 elicited elevated secretory (S)-IgA and serum IgG titers, and Salmonella-V-Ag(pV55) elicited much greater S-IgA and serum IgG Ab titers than Salmonella-V-Ag(pV3) or Salmonella-V-Ag(pV4). Hence, a new Salmonella vaccine, Salmonella-(F1+V)Ags, made with a single plasmid containing the caf1 operon and the chimeric promoter for V-Ag allowed the simultaneous expression of F1 capsule and V-Ag. Salmonella-(F1+V)Ags elicited elevated Ab titers similar to their monotypic derivatives. For bubonic plague, mice dosed with Salmonella-(F1+V)Ags and Salmonella-F1-Ag showed similar efficacy (&amp;gt;83% survival) against \u223c1000 LD50 \u00a0Y. pestis. For pneumonic plague, immunized mice required immunity to both F1- and V-Ags because the mice vaccinated with Salmonella-(F1+V)Ags protected against 100 LD50 \u00a0Y. pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis.<\/jats:p>","DOI":"10.4049\/jimmunol.178.2.1059","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:17:51Z","timestamp":1397848671000},"page":"1059-1067","source":"Crossref","is-referenced-by-count":57,"title":["Oral Vaccination with\n                    <i>Salmonella<\/i>\n                    Simultaneously Expressing\n                    <i>Yersinia pestis<\/i>\n                    F1 and V Antigens Protects against Bubonic and Pneumonic Plague"],"prefix":"10.1093","volume":"178","author":[{"given":"Xinghong","family":"Yang","sequence":"first","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B Joseph","family":"Hinnebusch","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Trunkle","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catharine M","family":"Bosio","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University , Fort Collins, CO 80521"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Suo","sequence":"additional","affiliation":[{"name":"Physics Department, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Tighe","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Harmsen","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Becker","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Crist","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Walters","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Recep","family":"Avci","sequence":"additional","affiliation":[{"name":"Physics Department, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W","family":"Pascual","sequence":"additional","affiliation":[{"name":"Veterinary Molecular Biology, Montana State University , Bozeman, MT 59717"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,1,15]]},"reference":[{"key":"2026012809421461700_R1","first-page":"35","volume-title":"Clin. 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