{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:55:56Z","timestamp":1790927756759,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The binding of soluble proteins to target surfaces is vital in triggering the immune response. However, structural insight into such processes is still lacking. Mannan-binding lectin (MBL) is a classic example of a pattern recognition molecule with important roles in innate immunity against microbial infections. By small angle x-ray scattering analysis we show that the large MBL complex in solution is folded into a ramified structure with a striking rotational symmetry and a structure permissive of elongation by unbending. Nevertheless, the structure in solution is found to be very stable. However, when the MBL molecule interacts with surface-immobilized ligands, the stable MBL structure is broken into a stretched state with separation of the ligand-binding domains as shown by high resolution atomic force microscopy. These studies provide a snapshot of the single molecule mechanics of MBL and the first direct evidence that the transition from the soluble state to surface-bound protein involves large conformational changes in the quaternary structure, thus highlighting the role of surface topography in immune recognition.<\/jats:p>","DOI":"10.4049\/jimmunol.178.5.3016","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T13:14:39Z","timestamp":1397826879000},"page":"3016-3022","source":"Crossref","is-referenced-by-count":50,"title":["Conformational Changes in Mannan-Binding Lectin Bound to Ligand Surfaces"],"prefix":"10.1093","volume":"178","author":[{"given":"Mingdong","family":"Dong","sequence":"first","affiliation":[{"name":"Interdisciplinary Nanoscience Center (iNANO), University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Department of Physics and Astronomy, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sailong","family":"Xu","sequence":"additional","affiliation":[{"name":"Interdisciplinary Nanoscience Center (iNANO), University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Department of Physics and Astronomy, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiano L P","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan S","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Interdisciplinary Nanoscience Center (iNANO), University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Department of Chemistry, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Thiel","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Immunology, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flemming","family":"Besenbacher","sequence":"additional","affiliation":[{"name":"Interdisciplinary Nanoscience Center (iNANO), University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Department of Physics and Astronomy, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Vorup-Jensen","sequence":"additional","affiliation":[{"name":"Interdisciplinary Nanoscience Center (iNANO), University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Biophysical Immunology Laboratory, University of Aarhus , Aarhus ,","place":["Denmark"]},{"name":"Institute for Medical Microbiology and Immunology, University of Aarhus , Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,3,1]]},"reference":[{"key":"2026012810512207700_R1","first-page":"197","volume-title":"Annu. 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