{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:18:53Z","timestamp":1791839933231,"version":"4.3.4"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pneumocystis carinii (PC) pneumonia is a leading opportunistic infection found among HIV-infected individuals worldwide. Although CD4+ T cell deficiency clearly correlates with susceptibility to PC pneumonia, murine models of disease indicate that PC-directed Abs may prevent infection and\/or inhibit growth of existing PC within the lungs. Recognition of PC by alveolar macrophages involves the \u03b2-glucan receptor Dectin-1 and macrophage effector function against PC is enhanced by Abs derived from PC-vaccinated hosts. We developed a fusion protein consisting of the extracellular domain of Dectin-1 linked to the Fc portion of murine IgG1, which we hypothesized would enhance host recognition and opsonic phagocytosis of PC. The recombinant protein, Dectin-Fc, is dimeric and the Ag recognition site identifies \u03b2-1,3 glucan linkages specifically and with high affinity (KD = 2.03 \u00d7 10\u22127 M). Dectin-Fc enhances RAW264.7 macrophage recognition of the \u03b2-glucan containing particulate zymosan in an Fc\u03b3RII- and Fc\u03b3RIII-dependent manner and preopsonization of PC organisms with Dectin-Fc increased alveolar and peritoneal macrophage-dependent killing of PC. SCID mice treated with a replication incompetent adenoviral vector expressing Dectin-Fc had attenuated growth of PC within the lungs, overall decreased PC lung burden, and diminished correlates of PC-related lung damage relative to SCID mice receiving a control vector. These findings demonstrate that targeting PC \u03b2-glucan with Dectin-Fc enhances host recognition and clearance of PC in the absence of B and T cells, and suggest that Fc\u03b3R-based targeting of PC, via cell wall carbohydrate recognition, may promote resistance against PC pneumonia in the immunodeficient host.<\/jats:p>","DOI":"10.4049\/jimmunol.178.6.3702","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:26:46Z","timestamp":1397849206000},"page":"3702-3712","source":"Crossref","is-referenced-by-count":42,"title":["Enhanced Defense against\n                    <i>Pneumocystis carinii<\/i>\n                    Mediated by a Novel Dectin-1 Receptor Fc Fusion Protein"],"prefix":"10.1093","volume":"178","author":[{"given":"Rekha R","family":"Rapaka","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"},{"name":"Department of Immunology, University of Pittsburgh School of Medicine , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S","family":"Goetzman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingquan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Vockley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"McKinley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay K","family":"Kolls","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"},{"name":"Department of Immunology, University of Pittsburgh School of Medicine , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad","family":"Steele","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pulmonary Medicine, Allergy, and Immunology and Division of Medical Genetics, Children\u2019s Hospital of Pittsburgh , Pittsburgh, PA 15213"},{"name":"Department of Immunology, University of Pittsburgh School of Medicine , Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,3,15]]},"reference":[{"key":"2026012809381230600_R1","first-page":"1713","volume-title":"Emerg. 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