{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:24:03Z","timestamp":1792185843218,"version":"4.4.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Neisseria gonorrhoeae and Neisseria meningitidis both express the lacto-N-neotetraose (LNT) lipooligosaccharide (LOS) molecule that can be sialylated. Although gonococcal LNT LOS sialylation enhances binding of the alternative pathway complement inhibitor factor H and renders otherwise serum-sensitive bacteria resistant to complement-dependent killing, the role of LOS sialylation in meningococcal serum resistance is less clear. We show that only gonococcal, but not meningococcal, LNT LOS sialylation enhanced factor H binding. Replacing the porin (Por) B molecule of a meningococcal strain (LOS sialylated) that did not bind factor H with gonococcal Por1B augmented factor H binding. Capsule expression did not alter factor H binding to meningococci that express gonococcal Por. Conversely, replacing gonococcal Por1B with meningococcal PorB abrogated factor H binding despite LNT LOS sialylation. Gonococcal Por1B introduced in the background of an unsialylated meningococcus itself bound small amounts of factor H, suggesting a direct factor H-Por1B interaction. Factor H binding to unsialylated meningococci transfected with gonococcal Por1B was similar to the sialylated counterpart only in the presence of higher (20 \u03bcg\/ml) concentrations of factor H and decreased in a dose-responsive manner by \u223c80% at 1.25 \u03bcg\/ml. Factor H binding to the sialylated strain remained unchanged over this factor H concentration range however, suggesting that LOS sialylation facilitated optimal factor H-Por1B interactions. The functional counterpart of factor H binding showed that sialylated meningococcal mutants that possessed gonococcal Por1B were resistant to complement-mediated killing by normal human serum. Our data highlight the different mechanisms used by these two related species to evade complement.<\/jats:p>","DOI":"10.4049\/jimmunol.178.7.4489","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T22:53:35Z","timestamp":1397861615000},"page":"4489-4497","source":"Crossref","is-referenced-by-count":71,"title":["Factor H Binding and Function in Sialylated Pathogenic Neisseriae is Influenced by Gonococcal, but Not Meningococcal, Porin"],"prefix":"10.1093","volume":"178","author":[{"given":"Guillermo","family":"Madico","sequence":"first","affiliation":[{"name":"Evans Biomedical Research Center, Boston University Medical Center , Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutamas","family":"Ngampasutadol","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Memorial Medical Center , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunita","family":"Gulati","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Memorial Medical Center , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Vogel","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, Universita\u0308t Wu\u0308rzburg , 97080 Wu\u0308rzburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A","family":"Rice","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Memorial Medical Center , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Ram","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Memorial Medical Center , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,4,1]]},"reference":[{"key":"2025032802441911900_R1","first-page":"638","volume-title":"Am. J. Med.","year":"1979"},{"key":"2025032802441911900_R2","first-page":"243","volume-title":"Medicine","year":"1984"},{"key":"2025032802441911900_R3","first-page":"98","volume-title":"Clin. Infect. Dis.","year":"1999"},{"key":"2025032802441911900_R4","first-page":"6299","volume-title":"J. Immunol.","year":"2005"},{"key":"2025032802441911900_R5","first-page":"281","volume-title":"J. Exp. Med.","year":"2001"},{"key":"2025032802441911900_R6","first-page":"671","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2025032802441911900_R7","first-page":"743","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2025032802441911900_R8","first-page":"1407","volume-title":"J. Gen. Microbiol.","year":"1986"},{"key":"2025032802441911900_R9","first-page":"303","volume-title":"Microb. Pathog.","year":"1988"},{"key":"2025032802441911900_R10","first-page":"39","volume-title":"Infect. Immun.","year":"1992"},{"key":"2025032802441911900_R11","first-page":"281","volume-title":"Antonie Leeuwenhoek.","year":"1995"},{"key":"2025032802441911900_R12","first-page":"506","volume-title":"J. Infect. Dis.","year":"1990"},{"key":"2025032802441911900_R13","first-page":"124","volume-title":"J. Infect. Dis.","year":"1999"},{"key":"2025032802441911900_R14","first-page":"228","volume-title":"13th International Pathogenic Neisseria Conference","year":"2002"},{"key":"2025032802441911900_R15","first-page":"2617","volume-title":"Mol. Microbiol.","year":"1992"},{"key":"2025032802441911900_R16","first-page":"7390","volume-title":"Infect. Immun.","year":"2005"},{"key":"2025032802441911900_R17","first-page":"317","volume-title":"Microb. Pathog.","year":"1989"},{"key":"2025032802441911900_R18","first-page":"4436","volume-title":"Infect. Immun.","year":"1997"},{"key":"2025032802441911900_R19","first-page":"219","volume-title":"Microb. Pathog.","year":"1992"},{"key":"2025032802441911900_R20","first-page":"159","volume-title":"Med. Microbiol. Immunol.","year":"1997"},{"key":"2025032802441911900_R21","first-page":"954","volume-title":"Infect. Immun.","year":"1999"},{"key":"2025032802441911900_R22","first-page":"1669","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1989"},{"key":"2025032802441911900_R23","first-page":"501","volume-title":"J. Immunol.","year":"2006"},{"key":"2025032802441911900_R24","first-page":"50853","volume-title":"J. Biol. Chem.","year":"2003"},{"key":"2025032802441911900_R25","first-page":"5939","volume-title":"Infect. Immun.","year":"1998"},{"key":"2025032802441911900_R26","first-page":"751","volume-title":"Infect. Immun.","year":"1981"},{"key":"2025032802441911900_R27","first-page":"994","volume-title":"Infect. Immun.","year":"1984"},{"key":"2025032802441911900_R28","first-page":"137","volume-title":"Methods Enzymol.","year":"1994"},{"key":"2025032802441911900_R29","first-page":"506","volume-title":"J. Infect. Dis.","year":"1990"},{"key":"2025032802441911900_R30","first-page":"989","volume-title":"Infect. Immun.","year":"1992"},{"key":"2025032802441911900_R31","first-page":"4703","volume-title":"J. Biol. Chem.","year":"1971"},{"key":"2025032802441911900_R32","first-page":"2849","volume-title":"J. Biol. Chem.","year":"1971"},{"key":"2025032802441911900_R33","first-page":"2898","volume-title":"J. Clin. Microbiol.","year":"2004"},{"key":"2025032802441911900_R34","first-page":"E38","volume-title":"Clin. Infect. Dis.","year":"2005"},{"key":"2025032802441911900_R35","first-page":"7566","volume-title":"J. Immunol.","year":"2006"},{"key":"2025032802441911900_R36","first-page":"236","volume-title":"J. Med. Microbiol.","year":"1994"},{"key":"2025032802441911900_R37","first-page":"2153","volume-title":"Mol. Microbiol.","year":"1991"},{"key":"2025032802441911900_R38","first-page":"377","volume-title":"Bacterial Outer Membranes as Model Systems","year":"1986"},{"key":"2025032802441911900_R39","first-page":"504","volume-title":"Rev. Infect. Dis.","year":"1985"},{"key":"2025032802441911900_R40","first-page":"8135","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1987"},{"key":"2025032802441911900_R41","first-page":"647","volume-title":"Mol. Microbiol.","year":"1998"},{"key":"2025032802441911900_R42","first-page":"3784","volume-title":"J. Immunol.","year":"2004"},{"key":"2025032802441911900_R43","first-page":"571","volume-title":"Cell Microbiol.","year":"2002"},{"key":"2025032802441911900_R44","first-page":"845","volume-title":"Clin. Diagn. Lab. Immunol.","year":"1998"},{"key":"2025032802441911900_R45","first-page":"13","volume-title":"J. Gen. Microbiol.","year":"1982"},{"key":"2025032802441911900_R46","first-page":"321","volume-title":"J. Infect. Dis.","year":"1973"},{"key":"2025032802441911900_R47","first-page":"45","volume-title":"Cell","year":"1982"},{"key":"2025032802441911900_R48","first-page":"1883","volume-title":"J. Exp. Med.","year":"1988"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/178\/7\/4489\/62659948\/zim00707004489.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/178\/7\/4489\/62659948\/zim00707004489.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T20:33:39Z","timestamp":1743366819000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/178\/7\/4489\/8023568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,1]]},"references-count":48,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,4,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.178.7.4489","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,4]]},"published":{"date-parts":[[2007,4,1]]}}}