{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:32:05Z","timestamp":1791221525188,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Differentiation of Ag-specific T cells into IFN-\u03b3 producers is essential for protective immunity to intracellular pathogens. In addition to stimulation through the TCR and costimulatory molecules, IFN-\u03b3 production is thought to require other inflammatory cytokines. Two such inflammatory cytokines are IL-12 and type I IFN (IFN-I); both can play a role in priming naive T cells to produce IFN-\u03b3 in vitro. However, their role in priming Ag-specific T cells for IFN-\u03b3 production during experimental infection in vivo is less clear. In this study, we examine the requirements for IL-12 and IFN-I, either individually or in combination, for priming Ag-specific T cell IFN-\u03b3 production after Listeria monocytogenes (Lm) infection. Surprisingly, neither individual nor combined defects in IL-12 or IFN-I signaling altered IFN-\u03b3 production by Ag-specific CD8 T cells after Lm infection. In contrast, individual defects in either IL-12 or IFN-I signaling conferred partial (\u223c50%) reductions, whereas combined deficiency in both IL-12 and IFN-I signaling conferred more dramatic (75\u201395%) reductions in IFN-\u03b3 production by Ag-specific CD4 T cells. The additive effects of IL-12 and IFN-I signaling on IFN-\u03b3 production by CD4 T cells were further demonstrated by adoptive transfer of transgenic IFN-IR+\/+ and IFN-IR\u2212\/\u2212 CD4 T cells into normal and IL-12-deficient mice, and infection with rLm. These results demonstrate an important dichotomy between the signals required for priming IFN-\u03b3 production by CD4 and CD8 T cells in response to bacterial infection.<\/jats:p>","DOI":"10.4049\/jimmunol.178.7.4498","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T22:53:35Z","timestamp":1397861615000},"page":"4498-4505","source":"Crossref","is-referenced-by-count":77,"title":["IL-12 and Type-I IFN Synergize for IFN-\u03b3 Production by CD4 T Cells, Whereas Neither Are Required for IFN-\u03b3 Production by CD8 T Cells after <i>Listeria monocytogenes<\/i> Infection"],"prefix":"10.1093","volume":"178","author":[{"given":"Sing Sing","family":"Way","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Havenar-Daughton","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Washington School of Medicine , Seattle, WA 98195"},{"name":"Washington National Primate Center, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesh A","family":"Kolumam","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Washington School of Medicine , Seattle, WA 98195"},{"name":"Washington National Primate Center, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nural N","family":"Orgun","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Washington School of Medicine , Seattle, WA 98195"},{"name":"Washington National Primate Center, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaja","family":"Murali-Krishna","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Washington School of Medicine , Seattle, WA 98195"},{"name":"Washington National Primate Center, University of Washington School of Medicine , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,4,1]]},"reference":[{"key":"2025032802524444200_R1","first-page":"5165","volume-title":"J. 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