{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:52:25Z","timestamp":1791100345908,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The E3-19K protein from human adenoviruses (Ads) retains class I MHC molecules in the endoplasmic reticulum. As a consequence, the cell surface expression of class I molecules is suppressed, allowing Ads to evade immune surveillance. Using native gel electrophoresis, gel filtration chromatography, and surface plasmon resonance, we show that a soluble form of the Ad type 2 (Ad2) E3-19K protein associates with HLA-A and -B molecules; equilibrium dissociation constants were in the nanomolar range and \u223c2.5-fold higher affinity for HLA-A (-A*0201, -A*0301, -A*1101, -A*3301, and -Aw*6801) relative to HLA-B (-B*0702 and -B*0801) molecules. Among the alleles of the HLA-A locus examined, HLA-A*3101 associated \u223c15-fold less avidly with soluble E3-19K. Soluble E3-19K interacted only very weakly with HLA-Cw*0304, and no interaction with HLA-Cw*0401 could be detected under identical conditions. Site-directed mutagenesis and flow cytometry demonstrated that MHC residue 56 plays a critical role in the association and endoplasmic reticulum retention of HLA-A molecules by E3-19K. This delineates the spatial environment around residue 56 as a putative E3-19K interaction surface on class I molecules. Overall, our data imply that a link may exist between host genetic factors and the susceptibility of individuals to Ad infections.<\/jats:p>","DOI":"10.4049\/jimmunol.178.7.4567","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T22:53:35Z","timestamp":1397861615000},"page":"4567-4575","source":"Crossref","is-referenced-by-count":25,"title":["Allele- and Locus-Specific Recognition of Class I MHC Molecules by the Immunomodulatory E3-19K Protein from Adenovirus"],"prefix":"10.1093","volume":"178","author":[{"given":"Hong","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy, University of Connecticut , Storrs, CT 06269"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Connecticut , Storrs, CT 06269"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Bouvier","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Connecticut , Storrs, CT 06269"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,4,1]]},"reference":[{"key":"2025032802554568800_R1","first-page":"1723","volume-title":"Virology","year":"1990"},{"key":"2025032802554568800_R2","first-page":"1","volume-title":"Virology","year":"1991"},{"key":"2025032802554568800_R3","first-page":"1679","volume-title":"Virology","year":"1990"},{"key":"2025032802554568800_R4","first-page":"5257","volume-title":"Proc. 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