{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:05:04Z","timestamp":1789758304040,"version":"4.0.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Autoimmune diabetes occurs when invading lymphocytes destroy insulin-producing \u03b2 cells in pancreatic islets. The role of lymphocytic aggregates at this inflammatory site is not understood. We find that B and T lymphocytes attacking islets in NOD mice organize into lymphoid structures with germinal centers. Analysis of BCR L chain genes was used to investigate selection of B lymphocytes in these tertiary lymphoid structures and in draining pancreatic lymph nodes. The pancreatic repertoire as a whole was found to be highly diverse, with the profile of L chain genes isolated from whole pancreas differing from that observed in regional lymph nodes. A V\u03ba14 L chain predominated within the complex pancreatic repertoire of NOD mice. Skewing toward V\u03ba4 genes was observed in the pancreas when the repertoire of NOD mice was restricted using a fixed Ig H chain transgene. Nucleotide sequencing of expressed V\u03bas identified shared mutations in some sequences consistent with Ag-driven selection and clonal expansion at the site of inflammation. Isolated islets contained oligoclonal B lymphocytes enriched for the germinal center marker GL7 and for sequences containing multiple mutations within CDRs, suggesting local T-B interactions. Together, these findings identify a process that selects B lymphocyte specificities within the pancreas, with further evolution of the selected repertoire at the inflamed site. This interpretation is reinforced by Ag-binding studies showing a large population of insulin-binding B lymphocytes in the pancreas compared with draining lymph nodes.<\/jats:p>","DOI":"10.4049\/jimmunol.178.9.5643","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:04:28Z","timestamp":1397847868000},"page":"5643-5651","source":"Crossref","is-referenced-by-count":98,"title":["Tertiary Lymphoid Structures in the Pancreas Promote Selection of B Lymphocytes in Autoimmune Diabetes"],"prefix":"10.1093","volume":"178","author":[{"given":"Peggy L","family":"Kendall","sequence":"first","affiliation":[{"name":"Departments of Medicine, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guowu","family":"Yu","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily J","family":"Woodward","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W","family":"Thomas","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Vanderbilt University , Nashville, TN 37232"},{"name":"Microbiology and Immunology, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,5,1]]},"reference":[{"key":"2026012809375350900_R1","first-page":"32","volume-title":"Arthritis Rheum.","year":"1984"},{"key":"2026012809375350900_R2","first-page":"650","volume-title":"J. 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